BONE AND CARTILAGE FORMATION IN DIFFUSION-CHAMBERS BY SUBCULTURED CELLS DERIVED FROM THE PERIOSTEUM
BONE AND CARTILAGE FORMATION IN DIFFUSION-CHAMBERS BY SUBCULTURED CELLS DERIVED FROM THE PERIOSTEUM
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DOI：
10.1016/8756-3282(90)90212-h
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发表时间：
1990-01-01
期刊：
影响因子：
4.1
通讯作者：
CAPLAN, AI
中科院分区：
文献类型：
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作者：
NAKAHARA, H;BRUDER, SP;CAPLAN, AI
从雏鸡胫骨中酶解分离骨膜细胞，引入细胞培养，使其达到合流，传代培养。将新分离或传代培养的细胞装入扩散室，植入胸腺小鼠腹膜腔，在体内封闭位置测试其成骨软骨潜能。新鲜分离的骨膜细胞在这样的实验室内形成骨和软骨组织，但发生率相对较低。相比之下，培养的骨膜细胞即使在数量翻倍10次后也能持续生长出骨和软骨。随着细胞的进一步传代，骨-软骨形成的潜力大大降低，直到16倍或更长时间的群体完全丧失表达能力。当将培养的肌肉成纤维细胞在与培养的骨膜细胞相同的条件下放入扩散室时，既不形成骨也不形成软骨。这些观察结果表明，雏鸡的骨膜细胞含有祖细胞或间充质干细胞亚群，这些细胞具有分化成成骨细胞或软骨细胞的潜力，并且这种潜力在酶分离和培养中几次群体加倍后仍保持不变。
Periosteal cells were enzymatically isolated from the tibiae of young chicks, introduced into cell culture, allowed to reach confluence, and subcultured. The freshly isolated or subcultured cells were loaded into diffusion chambers and implanted into the peritoneal cavity of athymic mice to test their osteo-chondrogenic potential in a contained in vivo location. Freshly isolated periosteal cells formed both bone and cartilage tissue in such test chambers, but with a relatively low incidence. In contrast, cultured periosteal cells consistently gave rise to bone and cartilage even after 10 population doublings. With further passages of cells, the osteo-chondrogenic potential diminished substantially, until complete loss of expressivity at 16 population doublings or longer. Cultured muscle fibroblasts, when loaded into diffusion chambers under identical conditions to those of cultured periosteal cells, formed neither bone nor cartilage. These observations suggest that periosteal cells of young chicks contain subsets of progenitor cells or mesenchymal stem cells which possess the potential to differentiate into osteoblasts or chondrocytes, and this potential is retained after enzymatic isolation and for several population doublings in culture.