pH Auxostat continuous culture of Acetobacteriumsp. coupled with removal of acetate by Methanosaeta concilii
pH Auxostat continuous culture of Acetobacteriumsp. coupled with removal of acetate by Methanosaeta concilii
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DOI：
10.1023/a:1018443200108
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发表时间：
1997-10
影响因子：
2.7
通讯作者：
A. Bainotti;K. Futagami;Y. Nakashimada;Y. Chang;S. Nagai;N. Nishio
中科院分区：
文献类型：
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作者：
A. Bainotti;K. Futagami;Y. Nakashimada;Y. Chang;S. Nagai;N. Nishio
一种新的醋酸菌连续培养系统。描述了与甲烷固定床反应器相耦合的装置。在甲醇(2 5 0mmoL L)+CO(D=0.0 2 h)条件下，在甲醇消耗量为83%的条件下，维生素B12的产量为1.2 mg L d，细胞产量为0.31g L d。然而，对于甲醇(250mmolL)-甲酸盐(150mmolL)，在完全消耗甲醇的情况下，在相同的D下，这些生产率分别提高了2.7%左右。当D=0.15h~(-1)时，发酵效率提高约14倍，分别为17 mg L·d和4.3g L·d，甲醇消耗量为51%。
A novel continuous culture system of Acetobacterium sp. coupled with a fixed-bed reactor of Methanosaeta conciliiis described. With methanol (250 mmol l ) plus CO at D= 0.02 h , the productivities of vitamin B 12 and cells were 1.2 mg l .d and 0.31 g l d , respectively, with 83% methanol consumption. With methanol (250 mmol l )-formate (150 mmol l ), however, these productivities increased ca. 2.7-fold, respectively at the same D, with complete consumption of methanol. At D= 0.15 h -1 with these latter substrates, these productivities enhanced ca. 14-fold, that is, 17 mg l .d and 4.3 g l .d, respectively, with 51% methanol consumption.