Chromosome aberrations induced by high-LET radiations.
Chromosome aberrations induced by high-LET radiations.
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DOI：
10.2187/bss.18.216
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发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Cucinotta, Francis A
中科院分区：
文献类型：
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作者：
Kawata, Tetsuya;Ito, Hisao;Cucinotta, Francis A
测量外周血淋巴细胞中的染色体畸变是目前可用于生物剂量测定的最灵敏和最可靠的辐射照射指标。这项技术最近已被用于研究宇航员在空间飞行期间遭受的辐射照射，其中很大一部分剂量是由高LET粒子照射提供的。传统的有丝分裂细胞的细胞遗传学损伤的评估方法收集在一个时间点曝光后，可能不适合测量高LET辐射的影响，由于激烈的细胞周期扰动和间期细胞死亡诱导这种类型的曝光。在这篇手稿中，我们回顾了用于研究高LET诱导的细胞遗传学效应的方法学的最新进展，并评估了化学诱导的染色体早熟凝聚（PCC）作为中期分析的替代方法的使用。发表的高LET辐射体外暴露的细胞遗传学效应的数据进行审查，沿着生物剂量测定结果，从宇航员后，短期或长期的空间任务。
Measurements of chromosome aberrations in peripheral blood lymphocytes are currently the most sensitive and reliable indicator of radiation exposure that can be used for biological dosimetry. This technique has been implemented recently to study radiation exposures incurred by astronauts during space flight, where a significant proportion of the dose is delivered by high-LET particle exposure. Traditional methods for the assessing of cytogenetic damage in mitotic cells collected at one time point after exposure may not be suitable for measuring high-LET radiation effects due to the drastic cell cycle perturbations and interphase cell death induced by this type of exposure. In this manuscript we review the recent advances in methodology used to study high-LET induced cytogenetic effects and evaluate the use of chemically-induced Premature Chromosome Condensation (PCC) as an alternative to metaphase analysis. Published data on the cytogenetic effects of in vitro exposures of high-LET radiation is reviewed, along with biodosimetry results from astronauts after short or long space missions.