The evolutionary history and tissue mapping of GPR123:: specific CNS expression pattern predominantly in thalamic nuclei and regions containing large pyramidal cells
The evolutionary history and tissue mapping of GPR123:: specific CNS expression pattern predominantly in thalamic nuclei and regions containing large pyramidal cells
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DOI：
10.1111/j.1471-4159.2006.04281.x
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发表时间：
2007-02-01
影响因子：
4.7
通讯作者：
Schioth, Helgi B.
中科院分区：
文献类型：
--
作者：
Lagerstrom, Malin C.;Rabe, Nadine;Schioth, Helgi B.
G蛋白偶联受体（GPCR）的粘附家族包括33种受体，并且是第二大GPCR家族。这些蛋白质中的大多数仍然是孤儿，对它们的组织分布和进化背景知之甚少。我们报告的进化历史的粘附家族蛋白GPR123以及GPR123的mRNA表达在小鼠和大鼠的原位杂交和实时PCR，分别映射。发现GPR123在脊椎动物谱系内非常保守，特别是在跨膜区和胞质尾区的远端部分，含有潜在的PDZ结合结构域。实时PCR数据表明GPR123主要在CNS中表达。原位数据显示在丘脑核和含有大锥体细胞的区域如皮质层5和6以及下托中高表达。此外，我们发现在杏仁核，下丘脑，下橄榄核和脊髓的表达明显。中枢神经系统的特异性表达，以及脊椎动物序列之间的高度保守性，表明GPR123可能在神经元信号转导的调节中起重要作用。
The Adhesion family of G protein-coupled receptors (GPCRs) includes 33 receptors and is the second largest GPCR family. Most of these proteins are still orphans and fairly little is known of their tissue distribution and evolutionary context. We report the evolutionary history of the Adhesion family protein GPR123 as well as mapping of GPR123 mRNA expression in mouse and rat using in situ hybridization and real-time PCR, respectively. GPR123 was found to be well conserved within the vertebrate lineage, especially within the transmembrane regions and in the distal part of the cytoplasmic tail, containing a potential PDZ binding domain. The real-time PCR data indicates that GPR123 is predominantly expressed in CNS. The in situ data show high expression in thalamic nuclei and regions containing large pyramidal cells like cortex layers 5 and 6 and subiculum. Moreover, we found distinct expression in amygdala, hypothalamus, inferior olive and spinal cord. The CNS specific expression, together with the high sequence conservation between the vertebrate sequences investigated, indicate that GPR123 may have an important role in the regulation of neuronal signal transduction.