Light/dark‐induced effects on behavioral rhythms in suprachiasmatic nucleus‐lesioned rats irrespective of the presence of functional suprachiasmatic nucleus brain implants
Light/dark‐induced effects on behavioral rhythms in suprachiasmatic nucleus‐lesioned rats irrespective of the presence of functional suprachiasmatic nucleus brain implants
复制标题
无论是否存在功能性视交叉上核脑植入物，光/暗对视交叉上核损伤大鼠行为节律的影响
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
W. Rietveld
中科院分区：
文献类型：
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作者：
G. Boer;H. Griffioen;H. Duindam;T. P. van der Woude;W. Rietveld
摘要视交叉上核(SCN)损毁的大鼠接受了胚胎SCN移植，连续三个月处于红光下。在这段时间之后，研究人员检查了那些表现出自由奔跑的昼夜节律恢复的大鼠是否可以被带入光/暗周期。为此，他们接受了12小时光照/12小时黑暗的时间表，然后是12小时的光照转换，然后再次处于黑暗条件下。此外，对没有恢复昼夜节律的SCN移植大鼠和有损伤的假移植动物也实施了同样的方案，作为对照进行研究。通过血管加压素、血管活性肠肽和生长抑素细胞的免疫细胞化学鉴定SCN移植物的存在。研究发现，在所有大鼠中，饮水、进食和跑轮的节律与光/暗周期同步，而不是与SCN移植物的存在同步。12小时的光移之后，紧接着是三个节律的移位。在最终的黑暗条件下，自由奔跑..。
Summary Suprachiasmatic nucleus (SCN)‐lesioned rats which had received a fetal SCN graft were kept in constant red light for three months. After this period it was examined whether those rats that showed a recovered free‐running circadian rhythm could be entrained to light/dark cycles. To this end, they were subjected to a 12 h light/12 h dark schedule, followed by a 12 h light shift and again to dark conditions. In addition, the same regime was imposed on SCN‐grafted rats without recovered circadian rhythms and on sham‐grafted animals with a lesion, which were studied as controls. The presence of an SCN graft was identified immunocytochemically by the presence of vasopressin, vasoactive intestinal polypeptide and somatostatin cells. Drinking, eating and wheel‐running rhythms were found to synchronize to the light/dark cycles in all rats, not with standing the presence of an SCN graft was. A 12 h light shift was immediately followed by a shift in the three rhythms. Under final dark conditions, free‐runnin...