Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries
Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries
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DOI：
10.2503/hortj.mi-041
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发表时间：
2015-07-01
影响因子：
1.2
通讯作者：
Yakushiji, Hiroshi
中科院分区：
文献类型：
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作者：
Azuma, Akifumi;Fujii, Hiroshi;Yakushiji, Hiroshi
葡萄（葡萄属）中黄酮类化合物的生物合成浆果表皮受环境因素如光和温度的影响。然而，光信号和低温诱导的脱落酸（阿坝）信号网络的组件相关的类黄酮积累在葡萄浆果的皮肤还没有完全阐明，以及环境条件如何影响这些组件仍然不清楚。为了阐明可能的光和ABA相关的信号转导网络的细节，我们开发了一个葡萄寡聚DNA微阵列（38,549个独立的探针），使用公开的葡萄基因组序列，并进行了全面的转录组分析，使用分离的“无”葡萄（葡萄属labruscana x V. vinifera）浆果培养在不同的光和温度条件下。利用基因芯片数据，我们探索了光诱导和低温诱导基因在葡萄果实中的变化。我们鉴定了40个光诱导基因、55个低温诱导基因和34个光加低温诱导基因。其中，我们选择伸长下胚轴5（HY 5），开放气孔1（OST 1），增强响应阿坝I（ERA 1）作为候选基因光诱导，低温诱导，光加低温诱导，分别。我们通过实时定量聚合酶链反应分析研究了它们在葡萄种质中的详细表达特征，并假设HY 5，OST 1和ERA 1可能通过光信号和低温信号网络参与类黄酮的生物合成。我们还建立了一个广泛的基因表达模式目录，以支持未来对葡萄浆果皮肤中对光和温度反应的其他候选基因的研究。
Flavonoid biosynthesis in grape (Vitis spp.) berry skin is affected by environmental factors such as light and temperature. However, the components of the light signaling and low-temperature-induced abscisic acid (ABA) signaling networks related to flavonoid accumulation in grape berry skin have not been fully elucidated, and how environmental conditions affect these components remains unclear. To clarify the details of the possible light- and ABA-related signal transduction networks, we developed a grape oligo-DNA microarray (38,549 independent probes) using the publicly available genomic sequence of grape, and performed comprehensive transcriptome analysis using detached 'None' grape (Vitis labruscana x V. vinifera) berries cultured under different light and temperature conditions. Using the microarray data, we explored the light-inducible and low-temperature-inducible genes in post-veraison grape berries. We identified 40 light-inducible genes, 55 low-temperature-inducible genes, and 34 genes induced by light plus low temperature. Among these, we selected elongated hypocotyl 5 (HY5), open stomata 1 (OST1), and enhanced response to ABA I (ERA1) as candidate light-inducible, low-temperature-inducible, and light- plus low-temperature-inducible genes, respectively. We investigated their detailed expression characteristics in grape accessions by means of quantitative real-time polymerase chain reaction analyses, and hypothesized that HY5, OST1, and ERA1 might be involved in flavonoid biosynthesis via light signaling and low-temperature signaling networks. We also established an extensive catalog of gene expression patterns to support future investigations of other candidate genes that respond to light and temperature in grape berry skin.