Soluble tumor necrosis factor receptors and interleukin-6 levels in patients with severe preeclampsia
Soluble tumor necrosis factor receptors and interleukin-6 levels in patients with severe preeclampsia
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DOI：
10.1016/0029-7844(96)00179-2
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发表时间：
1996-09-01
影响因子：
7.2
通讯作者：
Socol, ML
中科院分区：
文献类型：
--
作者：
Kupferminc, MJ;Peaceman, AM;Socol, ML
目的：为了探讨是否血清和羊水（AF）水平的可溶性肿瘤坏死因子受体和白细胞介素-6，免疫激活和内皮功能障碍的标志物，改变在重度子痫前期patients with severe preeclampsia.Methods：血浆收集引产前，分娩时，产后19例重度子痫前期。羊水也从这些患者的早期分娩中获得。相似的样本来自与胎龄匹配的产前对照组和无先兆子痫的足月对照组。所有的血浆和AF样本进行分析或p55和p75可溶性肿瘤坏死因子受体和白细胞介素-6的特异性酶联免疫测定。在先兆子痫患者和对照组的水平进行了compared.Results：两种受体的水平显着升高，在AF和所有产妇血浆样本，除了那些收集产后24小时的先兆子痫患者相对于对照组的水平。与对照组相比，先兆子痫患者在分娩前采集的血浆中更频繁地检测到白细胞介素-6，且浓度更高，但在分娩时白细胞介素-6的检测率或血浆浓度方面没有差异。相反，AF浓度的白细胞介素-6显着降低患者先兆子痫。结论：可溶性肿瘤坏死因子受体的水平增加，发现在重度先兆子痫患者可能代表一种保护性反应，以增加特纳坏死因子活性，是一个标志物免疫激活。分娩前母体血浆中白细胞介素-6浓度增加表明这种细胞因子也参与了免疫反应的改变及其对内皮细胞功能障碍的作用。
Objective: To investigate whether serum and amniotic fluid (AF) levels of soluble tumor necrosis factor receptors and interleukin-6, markers of immune activation and endothelial dysfunction, are altered in patients with severe preeclampsia.Methods: Plasma was collected before induction of labor, at delivery, and postpartum from 19 patients with severe preeclampsia. Amniotic fluid was also obtained in early labor from these patients. Similar samples were obtained from an antepartum control group matched for gestational age and a term control group without preeclampsia. All plasma and AF samples were assayed or p55 and p75 soluble tumor necrosis factor receptors and for interleukin-6 by specific enzyme-linked immunoassays. Levels in preeclamptic patients and the control groups were compared.Results: Levels of both receptors were significantly elevated in AF and all maternal plasma samples except those collected 24 hours postpartum for patients with preeclampsia relative to levels in controls. Interleukin-6 was detected more frequently and in higher concentrations in the plasma collected before labor for preeclamptic patients compared with controls, but no difference was noted in interleukin-6 detection rates or plasma concentrations at delivery. Conversely, AF concentrations of interleukin-6 were significantly reduced in patients with preeclampsia.Conclusion: The increased levels of soluble tumor necrosis factor receptors found in patients with severe preeclampsia may represent a protective response to increased turner necrosis factor activity and be a marker for immune activation. Increased interleukin-6 concentrations in maternal plasma before labor suggest the involvement of this cytokine as well in the altered immune response and its contribution to endothelial cell dysfunction.