Treatment response to isotretinoin correlates with specific shifts in Cutibacterium acnes strain composition within the follicular microbiome.
Treatment response to isotretinoin correlates with specific shifts in Cutibacterium acnes strain composition within the follicular microbiome.
复制标题
对异维A酸的治疗反应与毛囊微生物群内痤疮皮肤杆菌菌株组成的特定变化相关。
DOI：
10.1111/exd.14798
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发表时间：
2023
影响因子：
3.6
通讯作者：
Nelson,AmandaM
中科院分区：
文献类型：
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作者：
Nolan,ZacharyT;Banerjee,Kalins;Cong,Zhaoyuan;Gettle,SamanthaL;Longenecker,AmyL;Kawasawa,YukaI;Zaenglein,AndreaL;Thiboutot,DianeM;Agak,GeorgeW;Zhan,Xiang;Nelson,AmandaM
有没有药物作为有效的异维甲酸痤疮。解读成功治疗患者的毛囊皮脂腺中异维A酸诱导的微生物组变化可以为确定新的治疗方法铺平道路。我们确定了异维甲酸如何改变卵泡微生物组，并确定了哪些改变与成功的治疗反应相关。在异维A酸治疗前、治疗中和治疗后，对痤疮患者面部毛囊进行了全基因组测序。评估了微生物组的变化，并将其与20周时的治疗反应（定义为总体评估评分改善2级）相关。我们用计算方法研究了α多样性、β多样性、个体分类群的相对丰度、痤疮皮肤杆菌菌株组成和细菌代谢谱。我们发现，微生物组的β多样性增加与20周时对异维甲酸的成功治疗反应一致。异维A酸选择性改变C. SLST A和D群中的痤疮菌株多样性，D1菌株的多样性增加与成功的临床反应相关。异维A酸显著降低了与四种不同代谢途径相关的KEGG本体（KO）术语的患病率，从而推断滤泡微生物在治疗后的生长或存活能力可能有限。重要的是，在20周时未能实现成功应答的患者中未观察到微生物组成或代谢谱的这些改变。替代的方法来概括这种转变的平衡C。毛囊内的痤疮菌株和微生物组代谢功能可能对未来的痤疮治疗有益。
There are no drugs as effective as isotretinoin for acne. Deciphering the changes in the microbiome induced by isotretinoin in the pilosebaceous follicle of successfully treated patients can pave the way to identify novel therapeutic alternatives. We determined how the follicular microbiome changes with isotretinoin and identified which alterations correlate with a successful treatment response. Whole genome sequencing was done on casts from facial follicles of acne patients sampled before, during and after isotretinoin treatment. Alterations in the microbiome were assessed and correlated with treatment response at 20 weeks as defined as a 2‐grade improvement in global assessment score. We investigated the α‐diversity, β‐diversity, relative abundance of individual taxa,Cutibacterium acnesstrain composition and bacterial metabolic profiles with a computational approach. We found that increased β‐diversity of the microbiome coincides with a successful treatment response to isotretinoin at 20 weeks. Isotretinoin selectively alteredC. acnesstrain diversity in SLST A and D clusters, with increased diversity in D1 strains correlating with a successful clinical response. Isotretinoin significantly decreased the prevalence of KEGG Ontology (KO) terms associated with four distinct metabolic pathways inferring that follicular microbes may have limited capacity for growth or survival following treatment. Importantly, these alterations in microbial composition or metabolic profiles were not observed in patients that failed to achieve a successful response at 20 weeks. Alternative approaches to recapitulate this shift in the balance ofC. acnesstrains and microbiome metabolic function within the follicle may be beneficial in the future treatment of acne.