Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord
Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord
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DOI：
10.1117/1.3641988
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发表时间：
2011-10-01
影响因子：
3.5
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Shi, Yunzhou;Zhang, Delong;Cheng, Ji-Xin
白色物质的体内成像对于脱髓鞘的机制理解和髓鞘再生治疗的评价是重要的。虽然白色物质可以通过来自轴突髓鞘的强相干反斯托克斯拉曼散射（汽车）信号可视化，但是由于椎板切除术引起的复杂性，脊髓的体内重复汽车成像仍然是一个挑战。我们提出了一个仔细的实验设计，使纵向汽车成像的脱髓鞘和髓鞘再生在单个轴突水平的活大鼠。连续监测3周内分泌型磷脂酶A（2）诱导的髓鞘囊泡形成、髓鞘碎片的巨噬细胞摄取和许旺细胞的自发髓鞘再生的体内汽车成像。在单个轴突水平上的脱髓鞘和髓鞘再生的纵向可视化为旨在促进髓鞘可塑性和修复的治疗的合理设计提供了一个新的平台。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.3641988]
In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A(2) induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de-and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3641988]