Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes.
Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes.
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通过阳离子多层脂质体实现高效基因转移且细胞毒性较小。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
N. Ohishi
中科院分区：
文献类型：
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作者：
K. Yagi;H. Noda;M. Kurono;N. Ohishi
设计了一种制备阳离子多层囊泡（MLV）的简单方法，所述阳离子多层囊泡由N-（α-三甲基氨乙酰基）-双十二烷基-D-谷氨酸氯化物、双月桂酰磷脂酰胆碱和双油酰磷脂酰乙醇胺以1：2：2的摩尔比组成。当将噬菌体λ DNA包封到这些脂质体中时，发现包封率接近100%，并且DNA的消化率小于10%。在将质粒pCH 110封装到阳离子MLV中后，有效表达与通过反相蒸发法（REV）制备的阳离子囊泡获得的表达相当。本脂质体的细胞毒性低于阳离子REV，远低于Lipofectin。
A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.