Whole Genome Sequencing of the Braconid Parasitoid Wasp Fopius arisanus, an Important Biocontrol Agent of Pest Tepritid Fruit Flies.
Whole Genome Sequencing of the Braconid Parasitoid Wasp Fopius arisanus, an Important Biocontrol Agent of Pest Tepritid Fruit Flies.
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DOI：
10.1534/g3.117.040741
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发表时间：
2017-08-07
期刊：
影响因子：
--
通讯作者：
Sim SB
中科院分区：
文献类型：
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作者：
Geib SM;Liang GH;Murphy TD;Sim SB
茧蜂Fopiusjuanus（Sonan）是热带和亚热带害虫果蝇的重要生物控制剂，包括两种重要的全球性害虫，地中海实蝇（Ceratitiscapitata）和东方实蝇（Bactroceradorsalis）。本研究的目标是为该物种开发基础基因组资源，以提供工具，可用于回答探索宿主和寄生蜂之间的多营养相互作用在这个重要的研究系统的问题。在这里，我们提出了一个全基因组组装的F。来自一个未交配的雌性的单倍体后代池。基因组大小为154 Mb，其中N50重叠群和支架大小分别为51，867 bp和0.98 Mb。利用该物种现有的RNA-Seq数据，以及来自相关Hyplutera的公开可用的肽序列，生成了包括10，991个蛋白质编码基因的高质量基因注释集。在此组装提交之前，该物种不存在RefSeq蛋白。寄生蜂在多样化的生态系统中发挥着重要作用，并在农业害虫的生物防治中发挥作用。该全基因组组装和注释数据代表了该物种或任何密切相关的Opiine的第一个基因组规模组装，并且在国家生物技术信息中心基因组和RefSeq数据库中公开，为该hyperteran组提供了急需的基因组资源。
The braconid wasp Fopius arisanus (Sonan) is an important biological control agent of tropical and subtropical pest fruit flies, including two important global pests, the Mediterranean fruit fly (Ceratitis capitata), and the oriental fruit fly (Bactrocera dorsalis). The goal of this study was to develop foundational genomic resources for this species to provide tools that can be used to answer questions exploring the multitrophic interactions between the host and parasitoid in this important research system. Here, we present a whole genome assembly of F. arisanus, derived from a pool of haploid offspring from a single unmated female. The genome is ∼154 Mb in size, with a N50 contig and scaffold size of 51,867 bp and 0.98 Mb, respectively. Utilizing existing RNA-Seq data for this species, as well as publicly available peptide sequences from related Hymenoptera, a high quality gene annotation set, which includes 10,991 protein coding genes, was generated. Prior to this assembly submission, no RefSeq proteins were present for this species. Parasitic wasps play an important role in a diverse ecosystem as well as a role in biological control of agricultural pests. This whole genome assembly and annotation data represents the first genome-scale assembly for this species or any closely related Opiine, and are publicly available in the National Center for Biotechnology Information Genome and RefSeq databases, providing a much needed genomic resource for this hymenopteran group.