Pilocarpine-induced status epilepticus in mice: A comparison of spectral analysis of electroencephalogram and behavioral grading using the Racine scale.
Pilocarpine-induced status epilepticus in mice: A comparison of spectral analysis of electroencephalogram and behavioral grading using the Racine scale.
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DOI：
10.1016/j.eplepsyres.2015.09.008
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发表时间：
2015-11
影响因子：
2.2
通讯作者：
Zheng F
中科院分区：
文献类型：
--
作者：
Phelan KD;Shwe UT;Williams DK;Greenfield LJ;Zheng F
匹罗卡品诱导的癫痫持续状态（SE）是一种广泛应用的小鼠癫痫发作模型，拉辛量表已被用于表征癫痫发作强度。本研究的目的是利用快速傅立叶变换（FFT）对脑电图（EEG）进行定量分析，并统计评估癫痫发作与惊厥行为的相关性。同时对混合遗传背景的雄性小鼠进行脑电图和视频记录，并腹腔注射匹罗卡品诱导癫痫发作。录像采用拉辛量表评分，脑电图均方根（RMS）功率分析采用Sirenia癫痫发作Pro软件。我们发现RMS功率与匹罗卡平诱发的抽搐行为相关性非常弱。抽搐行为早在电痉挛发作前就出现了，并与θ频率活动呈强烈负相关，与γ频率活动呈中度正相关。在从第一次电痉挛到SE的过渡过程中，拉辛评分与多个频带的RMS功率有中等相关性。然而，在东南阶段，拉辛评分与RMS功率之间没有相关性，但在θ频率与RMS功率之间存在弱相关性。我们的分析揭示了拉辛量表作为癫痫持续状态中癫痫发作强度的主要指标的局限性，并表明需要对脑电图进行定量分析以准确评估癫痫发作和严重程度。
Pilocarpine-induced status epilepticus (SE) is a widely used seizure model in mice, and the Racine scale has been used to index seizure intensity. The goal of this study was to analyze electroencephalogram (EEG) quantitatively using fast Fourier transformation (FFT) and statistically evaluate the correlation of electrographic seizures with convulsive behaviors. Simultaneous EEG and video recordings in male mice in a mixed genetic background were conducted and pilocarpine was administered intraperitoneally to induce seizures. The videos were graded using the Racine scale and the root-mean-square (RMS) power analysis of EEG was performed with Sirenia Seizure Pro software. We found that the RMS power was very weakly correlated with convulsive behavior induced by pilocarpine. Convulsive behaviors appeared long before electrographic seizures and showed a strong negative correlation with theta frequency activity and a moderate positive correlation with gamma frequency activity. Racine scores showed moderate correlations with RMS power across multiple frequency bands during the transition from first electrographic seizure to SE. However, there was no correlation between Racine scores and RMS power during the SE phase except a weak correlation with RMS power in the theta frequency. Our analysis reveals limitations of the Racine scale as a primary index of seizure intensity in status epilepticus, and demonstrates a need for quantitative analysis of EEG for an accurate assessment of seizure onset and severity.