Bright Far-Red/Near-Infrared Conjugated Polymer Nanoparticles for In Vivo Bioimaging
Bright Far-Red/Near-Infrared Conjugated Polymer Nanoparticles for In Vivo Bioimaging
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DOI：
10.1002/smll.201300171
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发表时间：
2013-09-23
期刊：
影响因子：
13.3
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Ding, Dan;Liu, Jie;Liu, Bin
设计并合成了一种高发射性远红/近红外(FR/NIR)荧光共轭聚合物poly[(9，9-dihexylfluorene)-co-2，1，3-benzothiadiazole-co-4，7-di(thiophen-2-yl)-2，1，3-benzothiadiazole](PFBTDBT10)。以DSPE-PEG2000](DSPE-PEG2000)和DSPE-PEG5000-叶酸为包覆基质制备的PFBTDBT10纳米粒子(1，2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene-FA NPs)在水溶液中具有较强的红外/近红外荧光(27%量子产率)和较大的斯托克位移值233 nm。与市售量子点(Qdot655)和有机染料(如Alexa Fluor 555和罗丹明6G)相比，CPDP-FA纳米粒子表现出更好的热稳定性/光稳定性和更大的斯托克位移。在荷H22肝癌小鼠模型上的体内研究表明，CPDP-FA纳米粒可以作为一种高效的FR/NIR荧光探针，用于体内靶向荧光成像和高对比度和特异性的癌症检测。结合可忽略的体内毒性，CPDP-FA纳米粒有望成为未来在体内应用的FR/NIR荧光探针。
A highly emissive far-red/near-infrared (FR/NIR) fluorescent conjugated polymer (CP), poly[(9,9-dihexylfluorene)-co-2,1,3-benzothiadiazole-co-4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole] (PFBTDBT10) is designed and synthesized via Suzuki polymerization. Formulation of PFBTDBT10 using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG(2000)) and DSPE-PEG(5000)-folate as the encapsulation matrix yielded CP-loaded DSPE-PEG-folic acid nanoparticles (CPDP-FA NPs) with bright FR/NIR fluorescence (27% quantum yield) and a large Stoke's shift of 233 nm in aqueous solution. CPDP-FA NPs show improved thermal/photostabilities and larger Stoke's shifts as compared to commercially available quantum dots (Qdot 655) and organic dyes such as Alexa Fluor 555 and Rhodamine 6G. In vivo studies of CPDP-FA NPs on a hepatoma H22 tumor-bearing mouse model reveal that they could serve as an efficient FR/NIR fluorescent probe for targeted in vivo fluorescence imaging and cancer detection in a high contrast and specific manner. Together with the negligible in vivo toxicity, CPDP-FA NPs are promising FR/NIR fluorescent probes for future in vivo applications.