Relationship between fluoride exposure and osteoclast markers during RANKL-induced osteoclast differentiation
Relationship between fluoride exposure and osteoclast markers during RANKL-induced osteoclast differentiation
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DOI：
10.1016/j.etap.2016.08.001
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发表时间：
2016-09-01
影响因子：
4.3
通讯作者：
Sun Dianjun
中科院分区：
文献类型：
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作者：
Pei Junrui;Li Bingyun;Sun Dianjun
氟骨症是一种由氟化物过度积累引起的代谢性骨病。虽然这种疾病的病因已知，但氟化物在骨骼上积聚的机制尚未明确界定，因此在流行病学中没有可用于筛查氟骨症的标志物。本研究采用巨噬细胞集落刺激因子和核因子κ b受体激活剂配体处理C57BL/6小鼠骨髓细胞形成破骨细胞，采用实时荧光定量PCR （RT-PCR）检测抗酒石酸酸性磷酸酶5b （TRAP5b）、破骨细胞相关受体（OSCAR）、降钙素受体（CTR）、基质金属蛋白酶9 （MMP9）和组织蛋白酶K (CK) mRNA的表达。结果表明，0.5 ~ 8 mg/l的氟化物对破骨细胞的形成没有影响。0.5 mg/l的氟化物显著降低破骨细胞骨吸收活性。氟浓度与破骨细胞骨吸收活性呈负相关。在破骨细胞分化成熟第5天，MMP9和CK mRNA的表达不仅与氟浓度呈负相关，而且与破骨细胞骨吸收活性直接相关。TRAP5b、CTR、OSCAR mRNA表达量与破骨细胞数量呈正相关，与破骨细胞骨吸收活性无相关性。由此可见，MMP9和CK可能反映了骨吸收活性的变化以及氟暴露程度。TRAP5b、CTR、OSCAR能反映破骨细胞形成数量的变化。(C) 2016 Elsevier B.V.版权所有
Skeletal fluorosis is a metabolic bone disease caused by excessive accumulation of fluoride. Although the cause of this disease is known, the mechanism by which fluoride accumulates on the bone has not been clearly defined, thus there are no markers that can be used for screening skeletal fluorosis in epidemiology. In this study, osteoclasts were formed from bone marrow cells of C57BL/6 mice-treated with macrophage colony stimulating factor and receptor activator of nuclear factor kappa-B ligand: The mRNA expression of tartrate-resistant acid phosphatase 5b (TRAP5b), osteoclast-associated receptor (OSCAR), calcitonin receptor (CTR), matrix metalloproteinase 9 (MMP9) and cathepsin K (CK) were detected using real-time PCR (RT-PCR). Results showed that fluoride between 0.5 and 8 mg/l had no effect on osteoclast formation. However fluoride at 0.5 mg/l level significantly decreased the activity of osteoclast bone resorption. Fluoride concentration was negatively correlated with the activity of osteoclast bone resorption. On day 5 of osteoclast differentiation maturity, MMP9 and CK mRNA expression were not only negatively correlated with fluoride concentration, but directly correlated with the activity of osteoclast bone resorption. TRAP5b, CTR and OSCAR mRNA expression were positively correlated with the number of osteoclast and they had no correlation with the activity of osteoclast bone resorption. Thus, it can be seen that MMP9 and CK may reflect the change of activity of bone resorption as well the degree of fluoride exposure. TRAP5b, CTR and OSCAR can represent the change of number of osteoclast formed. (C) 2016 Elsevier B.V. All rights reserved.