Prevalence of human papillomaviruses (HPV) in benign and malignant tumors of the upper respiratory tract.
Prevalence of human papillomaviruses (HPV) in benign and malignant tumors of the upper respiratory tract.
复制标题
人乳头瘤病毒（HPV）在上呼吸道良性和恶性肿瘤中的患病率。
作者：
J. Shen;J. Tate;C. Crum;M. Goodman
人乳头瘤病毒（HPV）在头颈部肿瘤中的作用尚未确定。为了评估HPV在头颈部肿瘤中的可能作用，22例喉鳞状乳头状瘤（LP）、32例喉鳞状细胞癌（LSCC）、40例鼻内翻乳头状瘤（NIP）、14例鼻鳞状细胞癌（NSCC），应用聚合酶链反应（PCR）技术检测40例鼻咽癌（NPC）组织中HPVDNA的存在。HPV-L1共有引物、HPV 16和HPV 18 E7引物用于HPV核酸检测。采用L1 PCR产物的限制性片段长度多态性进行HPV分型。22例LP中18例（81.8%）、32例LSCC中3例（9.4%）、40例NIP中17例（42.5%）、14例NSCC中3例（21.4%）、40例NPC中均未检出HPV DNA。HPV 6在LP中的检出率高于HPV 11在NIP中的检出率（P < 0.001）。在3例HPV阳性的LSCC中，2例既往有LP，HPV 6和HPV 11阳性，既往有乳头状瘤。另1例HPV 18阳性。在NSCC患者中仅发现HPV 16。HPV阳性指数在伴NIP组（16.7%）与不伴NIP组（25.6%）之间无显著性差异。我们的研究结果表明，HPV在LP和NIP的发展中起作用，并且相似的病毒类型（即，HPV 6和HPV 11）可能在其组织特异性方面表现出相对差异。HPV在喉鳞状细胞癌和鼻咽癌病变中作为辅助因子的重要性有限，表明上呼吸道乳头状瘤和癌的发病机制存在差异。
The role for human papillomavirus (HPV) in head and neck tumors is not established. To evaluate the possible role of HPV in head and neck neoplasms, 22 cases of laryngeal squamous papilloma (LP), 32 cases of laryngeal squamous cell carcinoma (LSCC), 40 cases of nasal inverted papilloma (NIP), 14 cases of nasal squamous cell carcinoma (NSCC), and 40 cases of nasopharyngeal carcinoma (NPC) were evaluated for the presence of HPV DNA using the polymerase chain reaction (PCR) with two sets of primers in separate reactions: HPV-L1 consensus primers, HPV16 and HPV18 E7 primers for HPV nucleic acid detection. Restriction fragment length polymorphism of L1 PCR product was used for typing of HPV. Overall, HPV DNA was detected in 18 of 22 cases (81.8%) of LP, 3 of 32 cases (9.4%) of LSCC, 17 of 40 cases (42.5%) of NIP, 3 of 14 cases of NSCC (21.4%), and none of 40 cases of NPC. HPV6 was found more frequently in LP and HPV11 in NIP (P < 0.001). In the three HPV positive LSCCs, two cases had previous LP and were HPV6 and HPV11 positive, as were the prior papillomas. One other case was HPV18 positive. Only HPV16 was found in the NSCC patients. There was no significant difference in the index of HPV positivity between the NSCC cases associated with (16.7%) and without NIP (25.6%). Our results suggest that HPV plays a role in the development of both LP and NIP, and that similar viral types (i.e., HPV6 and HPV11) may exhibit relative differences in their tissue specificity. HPV appears to be of limited importance as a co-factor in LSCC and NPC lesions, indicating differences in the pathogenesis between papillomas and carcinomas in the upper respiratory tract.