Lysosomes and GERL in normal and chromatolytic neurons of the rat ganglion nodosum.
Lysosomes and GERL in normal and chromatolytic neurons of the rat ganglion nodosum.
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DOI：
10.1083/jcb.33.2.419
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发表时间：
1967-05
期刊：
影响因子：
--
通讯作者：
Villaverde H
中科院分区：
文献类型：
--
作者：
Holtzman E;Novikoff AB;Villaverde H
用大鼠结节性神经节研究轴突切断后的染色质溶解。在通过醛灌注固定后，将冷冻切片孵育用于作为细胞质细胞器的标记物的酶活性，如下：溶酶体的酸性磷酸酶和GERL（滑面内质网的高尔基体相关区域，溶酶体似乎从该区域发育）（31-33）;内质网和高尔基体的肌苷二磷酸酶;高尔基体的硫胺素焦磷酸酶;尼氏物质（内质网）的乙酰胆碱酯酶;线粒体的NADH-四-硝基BT还原酶。除了线粒体酶，所有的研究，电子显微镜以及光学显微镜。在染色质溶解的核周体中，细胞中心的粗面内质网被破坏，其余部分被分离到细胞周围。高尔基体、GERL、线粒体和溶酶体聚集在细胞的中央区域。GERL在正常和手术的细胞核中都很突出。电子显微镜图像显示其滑面内质网以几种方式产生各种溶酶体：（a）与内质网分离的包被囊泡;（B）由颗粒或膜状物质扩张的灶性区域产生的致密体;（c）囊泡和其他物质被隔离的“多囊体”;（d）含有内质网和核糖体的自噬空泡，可能来源于尼氏物质和线粒体。术后自噬泡数量增加。
The rat ganglion nodosum was used to study chromatolysis following axon section. After fixation by aldehyde perfusion, frozen sections were incubated for enzyme activities used as markers for cytoplasmic organelles as follows: acid phosphatase for lysosomes and GERL (a Golgi-related region of smooth endoplasmic reticulum from which lysosomes appear to develop) (31–33); inosine diphosphatase for endoplasmic reticulum and Golgi apparatus; thiamine pyrophosphatase for Golgi apparatus; acetycholinesterase for Nissl substance (endoplasmic reticulum); NADH-tetra-Nitro BT reductase for mitochondria. All but the mitochondrial enzyme were studied by electron microscopy as well as light microscopy. In chromatolytic perikarya there occur disruption of the rough endoplasmic reticulum in the center of the cell and segregation of the remainder to the cell periphery. Golgi apparatus, GERL, mitochondria and lysosomes accumulate in the central region of the cell. GERL is prominent in both normal and operated perikarya. Electron microscopic images suggest that its smooth endoplasmic reticulum produces a variety of lysosomes in several ways: (a) coated vesicles that separate from the reticulum; (b) dense bodies that arise from focal areas dilated with granular or membranous material; (c) "multivesicular bodies" in which vesicles and other material are sequestered; (d) autophagic vacuoles containing endoplasmic reticulum and ribosomes, presumably derived from the Nissl material, and mitochondria. The number of autophagic vacuoles increases following operation.