INTESTINAL TRANSPORT DURING THE LIFE-SPAN OF THE MOUSE
INTESTINAL TRANSPORT DURING THE LIFE-SPAN OF THE MOUSE
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DOI：
10.1093/geronj/45.4.b129
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发表时间：
1990-07-01
期刊：
影响因子：
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通讯作者：
WABNER, CL
中科院分区：
文献类型：
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作者：
CHEN, TS;CURRIER, GJ;WABNER, CL
肠道营养吸收不良可能是衰老的一个关键因素。因此，我们研究了不同年龄的小鼠的营养吸收情况，分别是生长期（3个月）、青壮年（12个月）、成熟期（24个月）、老年期（30个月）和老年期（33-36个月）。采用外翻囊技术研究了d -葡萄糖、3- o -甲基- d -葡萄糖（3MG）和l-酪氨酸的上皮转运。在老年小鼠中，生长过程中d -葡萄糖的上皮转运率增加了32%，随后下降了55%，3MG（一种非代谢的葡萄糖衍生物）也出现了同样的模式。然而，l -酪氨酸的上皮转运直到小鼠达到非常老的33-36个月时才受到损害。此外，通过乳酸生成测量的老年小鼠d -葡萄糖代谢率仅为成熟小鼠值的45%。此外，这些经上皮转运的改变伴随着绒毛数量和高度的减少。结果表明，随着年龄的增长，肠道上皮转运和吸收表面积减少。
Intestinal malabsorption of nutrients may be a critical factor in aging. For this reason, we investigated the nutrient absorption in mice of different ages of the life span, representing growth (3 mo), young adult (12 mo), mature (24 mo), old (30 mo), and very old (33-36 mo) periods. The everted sac technique was used to study the transepithelial transport of D-glucose, 3-O-methyl-D-glucose (3MG), and L-tyrosine. There was a 32% increase in the rate of D-glucose transepithelial transport during growth followed by a 55% decrease in the old mouse, and the same pattern occurred with 3MG, a nonmetabolized glucose derivative. However, L-tyrosine transepithelial transport was not impaired until the mouse reached the very old, 33-36 mo period. Also, the rate of D-glucose metabolism measured by lactate production in the very old mouse was only 45% of the mature mouse value. In addition, these transepithelial transport changes were accompanied by decreases in the number and the height of villi. The results indicate that intestinal transepithelial transport and the surface area for absorption decrease in aging.