Phosphatidylinositol phosphate, phosphatidylinositol bisphosphate, and the phosphoinositol sphingolipids are found in the plasma membrane and stimulate the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae.
Phosphatidylinositol phosphate, phosphatidylinositol bisphosphate, and the phosphoinositol sphingolipids are found in the plasma membrane and stimulate the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae.
复制标题
磷酸磷脂酰肌醇、二磷酸磷脂酰肌醇和磷酸肌醇鞘脂存在于质膜中，并刺激酿酒酵母的质膜H()-ATP酶。
DOI：
10.1016/0003-9861(92)90052-x
复制
发表时间：
1992
影响因子：
3.9
通讯作者：
Lester,RL
中科院分区：
文献类型：
--
作者：
Patton,JL;Lester,RL
研究了几种质膜磷脂对酿酒酵母质膜H ~+-ATP酶活性的调节作用。我们在这里表明，磷脂酰肌醇磷酸（PIP），磷脂酰肌醇二磷酸（PIP 2），和/或磷脂酰肌醇和PIP激酶主要位于质膜。以前的体内研究与S.科学家们已经表明PIP和PIP 2的水平发生了大的、快速的和可逆的变化，这与细胞ATP水平的变化是一致的。我们在这里证明，当分离的质膜被提供ATP或不被ATP洗涤时，它们在PIP和PIP 2含量上表现出相同的变化。利用混合胶束法，我们系统地研究了质膜脂质在维持质膜H+-ATP酶活性方面的功效。我们首次证明了一些质膜甘油磷脂有效地刺激ATP酶，包括PIP，PIP 2和心磷脂。含有肌醇的鞘脂是质膜的主要成分，也显示出以比甘油磷脂显著更低的水平刺激ATP酶，并且也必须被认为是体内重要的效应物。
Several plasma membrane phospholipids have been studied for their ability to modulate the activity of the plasma membrane H+-ATPase ofSaccharomyces cerevisiae. We show here that phosphatidylinositol phosphate (PIP), phosphatidylinositol bisphosphate (PIP2), and/or the phosphatidylinositol and PIP kinases are localized primarily in the plasma membrane. Previousin vivostudies withS. cerevisiaehave shown that large, rapid, and reversible changes occur in the levels of PIP and PIP2congruent with changes in cellular ATP levels. We demonstrate here that isolated plasma membranes exhibit the same changes in PIP and PIP2content when they are supplied with or washed free of ATP. Using a mixed micellar assay we systematically studied the efficacy of the plasma membrane lipids in sustaining the activity of the plasma membrane H+-ATPase. We demonstrate for the first time that a number of plasma membrane glycero-phospholipids effectively stimulate the ATPase, including PIP, PIP2, and cardiolipin. Phosphoinositol-containing sphingolipids, major components of the plasma membrane, are also shown to stimulate the ATPase at significantly lower levels than the glycerophospholipids and must also be considered as important effectorsin vivo.