Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing.
Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing.
复制标题
使用 16S rDNA 测序对供水系统中的异养细菌进行系统发育评估。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
P. Zalloua
中科院分区：
文献类型：
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作者：
S. Tokajian;Fuad Hashwa;I. Hancock;P. Zalloua
单独测定饮用水样品的异养菌平板计数（HPC）不足以评估与细菌计数突然变化相关的可能的健康危害。物种形成对于确定种群中是否包含病原体和（或）机会致病菌非常关键。大多数从饮用水样品中回收的菌株不能分配到一个特定的系统发育分支的基础上使用的常规诊断方法。本研究必须使用系统发育分析，这是通过确定和使用16 S rDNA的第一个500-bp序列简化，成功地确定样品中发现的细菌的类型和种类。革兰氏阳性菌α-、β-和γ-变形菌门被发现是代表饮用水中异养细菌的主要群体。该研究还表明，饮用水供应中存在鞘氨醇单胞菌可能比迄今为止报道的要普遍得多，因此值得进一步研究。供应的间歇模式，主要特点是水停滞和流动中断可能与生物膜分离，提出了这样的系统中的研究的细菌种群代表来自2个不同的生态位，生物膜和水柱的生物体的可能性。
Determination of a heterotrophic plate count (HPC) for drinking-water samples alone is not enough to assess possible health hazards associated with sudden changes in the bacterial count. Speciation is very crucial to determine whether the population includes pathogens and (or) opportunistic pathogens. Most of the isolates recovered from drinking water samples could not be allocated to a specific phylogenetic branch based on the use of conventional diagnostic methods. The present study had to use phylogenetic analysis, which was simplified by determining and using the first 500-bp sequence of the 16S rDNA, to successfully identify the type and species of bacteria found in the samples. Gram-positive bacteria alpha-, beta-, and gamma-Proteobacteria were found to be the major groups representing the heterotrophic bacteria in drinking water. The study also revealed that the presence of sphingomonads in drinking water supplies may be much more common than has been reported so far and thus further studies are merited. The intermittent mode of supply, mainly characterized by water stagnation and flow interruption associated possibly with biofilm detachment, raised the possibility that the studied bacterial populations in such systems represented organisms coming from 2 different niches, the biofilm and the water column.
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR