Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria.
Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria.
复制标题
紫色非硫光合细菌中蒎烯的生物合成。
DOI：
10.1186/s12934-021-01591-6
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发表时间：
2021-05-17
影响因子：
6.4
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Wu X;Ma G;Liu C;Qiu XY;Min L;Kuang J;Zhu L
蒎烯是一种单萜，用于制造香料，杀虫剂，精细化学品和可再生燃料。利用代谢工程微生物技术生产蒎烯是一种可持续发展的方法。紫色非硫光合细菌属于光合细菌，广泛应用于天然化学物质的合成。目前，紫色非硫光合细菌合成蒎烯的研究还未见报道，紫色非硫光合细菌合成蒎烯的潜力有待进一步开发。以球形红细菌（Rhodobacter sphaeroides）为模型菌株，通过基因工程技术，表达异源香叶基二磷酸合酶（GPPS）和蒎烯合酶（PS）的融合蛋白，实现了蒎烯的生产。优化了反应条件，获得了97.51 μ g/L的蒎烯产量。在此基础上，过量表达了1-脱氧-d-木酮糖5-磷酸合成酶、1-脱氧-d-木酮糖5-磷酸还原异构酶和异戊烯基二磷酸异构酶基因，并优化了GPPS-PS mRNA的核糖体结合位点，使蒎烯效价提高到539.84 μ g/L。 本论文通过外源表达GPPS-PS，成功地在R.通过优化关键酶的表达，可显著提高松油烯的产量。本研究首次报道了紫色非硫光合细菌产蒎烯的研究，为进一步利用紫色非硫光合细菌产蒎烯提供了新的光合底物。在线版本包含补充材料，可通过10.1186/s12934 - 021 - 01591 - 6获得。
Pinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Purple non-sulfur photosynthetic bacteria belong to photosynthetic chassis that are widely used to synthesize natural chemicals. To date, researches on the synthesis of pinene by purple non-sulfur photosynthetic bacteria has not been reported, leaving the potential of purple non-sulfur photosynthetic bacteria synthesizing pinene unexplored. Rhodobacter sphaeroides strain was applied as a model and engineered to express the fusion protein of heterologous geranyl diphosphate synthase (GPPS) and pinene synthase (PS), hence achieving pinene production. The reaction condition of pinene production was optimized and 97.51 μg/L of pinene was yielded. Then, genes of 1-deoxy-d-xylulose 5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase and isopentenyl diphosphate isomerase were overexpressed, and the ribosome binding site of GPPS-PS mRNA was optimized, improving pinene titer to 539.84 μg/L. In this paper, through heterologous expression of GPPS-PS, pinene was successfully produced in R. sphaeroides, and pinene production was greatly improved by optimizing the expression of key enzymes. This is the first report on pinene produce by purple non-sulfur photosynthetic bacteria, which expands the availability of photosynthetic chassis for pinene production. The online version contains supplementary material available at 10.1186/s12934-021-01591-6.
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影响因子：
3.5
作者：
Miller, B;Heuser, T;Zimmer, W
通讯作者：
Zimmer, W
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
7.2
作者：
Wang, Xueqing;Wu, Xiaomin;Guo, Liejin
通讯作者：
Guo, Liejin
影响因子：
4.7
作者：
Tashiro, Miki;Kiyota, Hiroshi;Umeno, Daisuke
通讯作者：
Umeno, Daisuke
影响因子：
4.7
作者：
Sarria, Stephen;Wong, Betty;Peralta-Yahya, Pamela
通讯作者：
Peralta-Yahya, Pamela