First report of two foodborne Salmonella enterica subsp. enterica serovar Bovismorbificans isolates carrying a novel mega-plasmid harboring blaDHA-1 and qnrB4 genes
First report of two foodborne Salmonella enterica subsp. enterica serovar Bovismorbificans isolates carrying a novel mega-plasmid harboring blaDHA-1 and qnrB4 genes
复制标题
首次报告两种食源性沙门氏菌肠亚种。
DOI：
10.1016/j.ijfoodmicro.2021.109439
复制
发表时间：
2021-10-20
影响因子：
5.4
通讯作者：
Shi, Lei
中科院分区：
文献类型：
--
作者：
Li, Lili;Olsen, Rikke Heidemann;Shi, Lei
肠炎沙门氏菌对广谱头孢菌素和氟喹诺酮类药物具有耐药性，已被世界卫生组织（WHO）列为高优先级病原体。虽然CTX-M型获得性β -内酰胺酶已在肠链球菌血清型牛瘟菌中检测到，但DHA酶在牛瘟链球菌中很少报道。在这项研究中，我们首次报道了两株多重耐药（MDR）牛链球菌（S. bovismorificans）共携带质粒编码AmpC (pAmpC) β -内酰胺酶基因（bla(dha1)）和qnrB基因，分别从鸡肉样品中分离到16Sal017和从中国广州零售市场的草鱼样品中分离到16Sal018。这两个菌株的bla（DHA-1）和qnrB基因都位于同一个新的217,773 bp的属于ST2的IncHI2质粒上。该质粒含有16个额外获得的耐药基因，编码对8类抗生素和季铵盐化合物的抗性。此外，16Sal017还含有一个10,124 bp的Col （pHAD28）样质粒，内含qnrS1。bla（DHA-1）和qnrB4基因位于sul1-qacE Delta 1- ampr -bla(DHA-1)-pspABCDF-qnrB4-sapABC-IS91-sul1-qacE Delta 1这一18,198 bp的区域，该区域已在多种细菌中被发现，表明bla（DHA-1）和qnrB4基因在该基因簇内具有较高的转移能力。发现IncHI2质粒可通过偶联转移到大肠杆菌J53上，并在转偶联物中获得多重抗性。cgMLST和MAUVE比对显示，16Sal017和16Sal018的基因组序列高度相似，与其他来源和国家的菌株没有流行病学上的联系。我们的研究结果表明，牛病葡萄球菌是携带bla（DHA-1）和qnrB4基因的结合质粒的新宿主，并强调了这些牛病葡萄球菌克隆通过食物链传播的潜在机会，需要继续研究。
Salmonella enterica displaying resistance to extended-spectrum cephalosporins and fluoroquinolone (FQs) has been deemed a high-priority pathogen by the World Health Organization (WHO). While CTX-M type acquired beta-lactamases have been detected in S. enterica serovar Bovismorbificans, DHA enzymes have been rarely reported in S. Bovismorbificans. In this study, we here report for the first time the isolation of two multi-drug resistant (MDR) S. Bovismorbificans strains co-harboring plasmid-encoded AmpC (pAmpC) beta-lactamase gene (bla(DHA-1)) and qnrB gene, 16Sal017 isolated from a chicken meat sample and 16Sal018 from a grass carp fish sample, collected from retail markets in Guangzhou, China. The bla(DHA-1) and qnrB genes in these two strains were both located on the same novel 217,773 bp IncHI2 plasmid belonged to ST2. The plasmid contained 16 additional acquired antimicrobial resistance genes encoding resistance to eight antibiotic classes and quaternary ammonium compound. Besides, 16Sal017 contained an additional 10,124 bp Col (pHAD28)-like plasmid harboring qnrS1. The bla(DHA-1) and qnrB4 genes were located in an 18,198 bp region, sul1-qacE Delta 1-ampR-bla(DHA-1)-pspABCDF-qnrB4-sapABC-IS91-sul1-qacE Delta 1, which has been identified in various bacteria species, indicating the high transfer ability of bla(DHA-1) and qnrB4 genes within this gene cluster. The IncHI2 plasmid was found to be transferable to Escherichia coli J53 by conjugation and resulted in the acquiring of multiple resistance in the transconjugants. Genome sequence comparisons by cgMLST and MAUVE alignment indicated 16Sal017 and 16Sal018 are highly similar and are not epidemiologically linked with strains from other sources and countries. Our findings suggest S. Bovismorbificans as a new host for conjugative mega-plasmid harboring bla(DHA-1) and qnrB4 genes, and highlight the potential transmission opportunity of these S. Bovismorbificans clones through the food chain, which need continuous investigation.