Electrophoretic karyotype of Aspergillus nidulans.
Electrophoretic karyotype of Aspergillus nidulans.
复制标题
构巢曲霉的电泳核型。
DOI：
10.1073/pnas.86.16.6260
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Carbon,J
中科院分区：
文献类型：
--
作者：
Brody,H;Carbon,J
采用等高线钳制的均匀电场凝胶电泳法，获得了尼杜拉曲霉的核型。共分离出6条染色体带，其中2条带以双重体的形式迁移。以裂殖酵母和酿酒酵母的染色体为大小标准，我们估计它们的染色体大小在2.9到5.0兆碱基对之间，总基因组大小约为31mb。通过轮廓钳制的均匀电场凝胶印迹与不同的标记探针进行杂交，将8个遗传连锁组中的4个分配给染色体带，每个标记的探针都针对特定的连锁组。对易位菌株的等高线夹持均匀电场凝胶分析给出了其余四个连锁群的染色体分配。按大小排序为：VIII(5.0mb)，VII(4.5mb)，II(4.2mb)，I和V(3.8mb)，III和VI(3.5mb)，IV(2.9mb)。
An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, we estimate the sizes of the chromosomes to be between 2.9 and 5.0 megabase pairs (mb) with a total genome size of approximately 31 mb. Four of the eight genetic linkage groups were assigned to chromosomal bands by hybridization of contour-clamped homogeneous electric field gel blots with various radiolabeled probes each specific to a particular linkage group. Contour-clamped homogeneous electric field gel analysis of reciprocal translocation strains gave chromosomal assignments for the four remaining linkage groups. In order of decreasing size, the A. nidulans chromosomes are: VIII (5.0 mb), VII (4.5 mb), II (4.2 mb), I and V (3.8 mb), III and VI (3.5 mb), and IV (2.9 mb).