A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head
A cytokine secreted from the suboesophageal body is essential for morphogenesis of the insect head
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DOI：
10.1016/j.mod.2004.10.005
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发表时间：
2005-02
影响因子：
2.6
通讯作者：
S. Tsuzuki;Shiroh Sekiguchi;M. Kamimura;M. Kiuchi;Y. Hayakawa
中科院分区：
文献类型：
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作者：
S. Tsuzuki;Shiroh Sekiguchi;M. Kamimura;M. Kiuchi;Y. Hayakawa
昆虫食道下体早在一个多世纪前就在蚕胚中被发现，但其生物学功能仍不清楚。我们发现该组织在卷心菜粘虫的早期胚胎阶段分化，并在产卵后24至60小时短暂分泌昆虫细胞因子生长阻断肽（GBP），此时正在进行原肠胚形成。通过显微注射由巨细胞病毒（CMV）组成启动子驱动的GBP基因实现GBP的过表达，导致头（胚胎头）的复杂畸形。用GBP双链RNA处理GBP，使胚胎中GBP的表达沉默，产生了严重的头部结构异常表型：含脑头的视叶减少，完全分离成双侧半。这表明食管下体分泌的GBP在胚胎发生早期头区形成中起重要作用。细胞因子诱导的双侧头叶融合被认为至少在昆虫中是进化保守的，因为昆虫胚胎中广泛存在食道下体。
The suboesophageal body of insects was identified over a century ago in the silkworm embryo, but its biological function is still unknown. We discovered that this tissue is differentiated in the earliest embryonic stages of the cabbage armyworm and secretes the insect cytokine, growth-blocking peptide (GBP), transiently from 24 to 60h after oviposition when gastrulation is in progress. Over-expression of GBP, achieved by microinjection of the GBP gene driven by a cytomegalovirus (CMV) constitutive promoter, resulted in complex deformities of the procephalon (embryonic head). Severe abnormal phenotypes of the head structure were produced by silencing the GBP expression in the embryo by treating with GBP double-stranded RNA: the procephalon-containing optic lobes diminished and completely separated into bilateral halves. This indicates that GBP secreted from the suboesophageal body plays an essential role in the formation of the procephalic domain during early embryogenesis. The cytokine-induced fusion of bilateral procephalic lobes is thought to be evolutionarily conserved at least in insects, because of the widespread occurrence of the suboesophageal body in insect embryos.