CLEAVAGE SITE DETERMINANTS IN THE MAMMALIAN POLYADENYLATION SIGNAL
CLEAVAGE SITE DETERMINANTS IN THE MAMMALIAN POLYADENYLATION SIGNAL
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DOI：
10.1093/nar/23.14.2614
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发表时间：
1995-07-25
影响因子：
14.9
通讯作者：
WILUSZ, J
中科院分区：
文献类型：
--
作者：
CHEN, F;MACDONALD, CC;WILUSZ, J
使用SV40晚期聚腺苷酸化信号的一系列位置和核苷酸变体，我们已经证明了三个序列元素确定了哺乳动物前MRNA中3'-末端切割的精确位点五个核苷酸，其中至少四个是尿苷，并且在裂解位点处的核苷酸偏好比g大得多，裂解的发生不到11个碱基，但距离AAUAAAA的23个碱基远不止23个碱基。元素。下游富含U的元件通常位于裂解位点，AAUAAA的相对位置，U-RICH元素的相对位置定义了将发生裂解的13个基本结构域内的近似区域。然后，裂解的确切位置由上述偏好顺序的局部核苷酸序列确定，该模型是调查了接近四分之三的聚腺苷酸化信号，并且与先前的实验观测一致。
Using a series of position and nucleotide variants of the SV40 late polyadenylation signal we have demonstrated that three sequence elements determine the precise site of 3'-end cleavage in mammalian pre-mRNAs: an upstream AAUAAA element, a downstream U-rich element consisting of five nucleotides, at least four of which are uridine, and a nucleotide preference at the site of cleavage in the order A > U > C much greater than G, Cleavage occurs no closer than 11 bases, but no farther than 23 bases from the AAUAAA element. The downstream U-rich element is usually located 10-30 bases from the cleavage site, The relative position of the AAUAAA and the U-rich elements define the approximate region within a 13 base domain in which cleavage will occur. The exact position of cleavage is then determined by the local nucleotide sequence in the order of preference noted above, This model accounts for nearly three quarters of polyadenylation signals surveyed and is consistent with previous experimental observations.