A microfluidic cartridge to prepare spores for PCR analysis
A microfluidic cartridge to prepare spores for PCR analysis
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DOI：
10.1016/s0956-5663(99)00060-3
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发表时间：
2000-01-01
影响因子：
12.6
通讯作者：
Northrup, MA
中科院分区：
文献类型：
--
作者：
Belgrader, P;Okuzumi, M;Northrup, MA
描述了一种原型盒系统，通过超声波快速破坏芽孢杆菌孢子，将PCR试剂添加到破坏的孢子中。并将混合物分配到PCR管中。自动处理盒中的孢子，然后通过实时PCR检测孢子DNA的总时间为20分钟。(C) 2000 Elsevier Science S.A.版权所有
A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores. and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min. (C) 2000 Elsevier Science S.A. All rights reserved.