Addition of β-mercaptoethanol or Trolox® at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents
Addition of β-mercaptoethanol or Trolox® at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents
复制标题
DOI：
10.1016/s0093-691x(03)00191-2
复制
发表时间：
2004-01-01
期刊：
影响因子：
2.8
通讯作者：
Donnay, I
中科院分区：
文献类型：
--
作者：
Feugang, JM;De Roover, R;Donnay, I
本研究旨在评价β-巯基乙醇（谷胱甘肽合成的刺激剂）和Trolox（R）（维生素E的水溶性类似物）对在氧化应激条件下从桑椹胚阶段（授精后第5天; pi）培养的牛胚胎的影响。用增加剂量的Trolox（R）培养胚胎在接种后第8天显示出剂量依赖性胚胎毒性。使用400 μ M Trolox以及100 μ M β-巯基乙醇在第8天至少部分防止了（P < 0.05）促氧化剂诱导的胚泡变性。抗氧化剂和β-巯基乙醇均能显著提高存活囊胚的孵化率，且单独使用β-巯基乙醇能显著提高存活囊胚的平均细胞数，其中以ICM的效果最为显著（P < 0.05）。对它们在第7天的作用的分析表明，两种抗氧化剂均显著减少促氧化剂诱导的细胞凋亡，β-巯基乙醇降低了细胞凋亡的生理水平，并刺激谷胱甘肽的合成（P < 0.05）。此外，体外和体内生产的胚胎之间的比较表明，在授精后相同年龄（桑椹胚和囊胚），细胞凋亡水平相似，但在体外胚胎中，细胞凋亡水平随着胚胎年龄的增加而稳定增加。总之，在桑椹胚阶段单独添加β-巯基乙醇和Trolox（R）可以保护胚胎免受氧化应激，并提高所得囊胚的质量。(C)2003年爱思唯尔公司All rights reserved.
This study was conducted to evaluate the effect of beta-mercaptoethanol (a stimulator of glutathione synthesis) and Trolox(R) (an hydrosoluble analogue of Vitamin E) on bovine embryos cultured from the morula stage (Day 5 post-insemination; pi) under oxidative stress conditions. Culture of embryos with increased doses of Trolox(R) showed a dose-dependent embryotoxicity on Day 8 pi. The use of 400 muM Trolox(R) as well as beta-mercaptoethanol at 100 muM prevented at least partly (P < 0.05) the prooxidant-induced blastocyst degeneration on Day 8. Hatching rates of surviving blastocysts were significantly increased by both antioxidants and β-mercaptoethanol alone improved their mean cell numbers, which was significant in the ICM (P < 0.05). Analysis of their effect on Day 7 pi showed that both the antioxidants significantly reduced the prooxidant-induced apoptosis and beta-mercaptoethanol diminished the physiological level of apoptosis as well as it stimulated the glutathione synthesis (P < 0.05). In addition, a comparison between in vitro- and in vivo-produced embryos showed that the levels of apoptosis were similar at the same age post-insemination (morulae and blastocysts) but increased steadily with the embryonic age in in vitro ones.In conclusion, β-mercaptoethanol and Trolox(R) added separately from the morula stage protected embryos against oxidative stress and improved the quality of the resulting blastocysts. (C) 2003 Elsevier Inc. All rights reserved.