Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites
Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites
复制标题
基于功能化石墨烯纳米复合材料的用于凝血酶检测的无标记适体生物传感器
DOI：
10.1016/j.talanta.2015.04.012
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发表时间：
2015
期刊：
影响因子：
6.1
通讯作者：
Dong Shaojun
中科院分区：
文献类型：
--
作者：
Wang Qingqing;Zhou Zhixue;Zhai Yanling;Zhang Lingling;Hong Wei;Zhang Zhiquan;Dong Shaojun
开发了一种基于功能化石墨烯纳米复合材料（rGO-AuNPs）的无标记和放大的电化学阻抗适配体传感器，用于检测凝血酶，凝血酶在血栓形成和止血中起着至关重要的作用。首先将巯基化的适配体和二硫苏糖醇（TBA 15-DTT）固定在金电极上捕获凝血酶分子，然后将适配体功能化的石墨烯纳米复合材料（rGO-TBA 29）制成三明治传感平台，用于放大阻抗信号。由于TBA 29在电极上的大量负电荷排斥[Fe（CN）6]4−/3−阴离子，导致明显放大的电荷转移电阻（Rct）信号。凝血酶浓度在0.3 ~ 50 nM范围内与Rct呈线性关系，检测限为0.01 nM。此外，石墨烯还可以通过静电或π-π堆积相互作用与其他探针标记产生信号，因此可以在该模型中使用不同的检测方法，扩大应用范围。
A label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rGO–AuNPs) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. The thiolated aptamer and dithiothreitol (TBA15–DTT) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rGO–TBA29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. As numerous negative charges of TBA29 on the electrode repelled to the [Fe(CN)6]4−/3−anions, resulting in an obvious amplified charge-transfer resistance (Rct) signal. TheRctincrease was linearly proportional to the thrombin concentration from 0.3 to 50 nM and a detection limit of 0.01 nM thrombin was achieved. In addition, graphene could also be labeled with other probes via electrostatic or π–π stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model.