EXPERIMENTAL TRANSMISSION OF BOVINE LEUKOSIS VIRUS BY SIMULATED RECTAL PALPATION
EXPERIMENTAL TRANSMISSION OF BOVINE LEUKOSIS VIRUS BY SIMULATED RECTAL PALPATION
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DOI：
10.1136/vr.122.16.389
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发表时间：
1988-04-16
影响因子：
2.2
通讯作者：
BANGERT, RL
中科院分区：
文献类型：
--
作者：
HOPKINS, SG;EVERMANN, JF;BANGERT, RL
8头6月龄荷斯坦公牛犊通过直肠触诊用来自牛白血病病毒血清阳性供体的全血进行实验性接种。接种包括将2ml全血沉积在一次性产科套管上，随后进行30秒直肠触诊以模拟妊娠检测或人工授精的过程。连续三周每周重复该程序。所有8头小牛在初次触诊后5周内均产生了牛白血病病毒抗体。在9周时，在所有8头小牛的外周血白细胞中均证实存在病毒。这些结果表明，常规直肠触诊可能是牛白血病病毒在易感牛中传播的一种有效方式。
Eight six-month-old Holstein male calves were experimentally inoculated by rectal palpation with whole blood from a donor seropositive to bovine leukosis virus. The inoculation consisted of the deposition of 2 ml of whole blood on a disposable obstetrical sleeve followed by a 30 second rectal palpation to simulate the process of pregnancy detection or artificial insemination. This procedure was repeated at weekly intervals for three consecutive weeks. All eight calves developed antibodies to bovine leukosis virus within five weeks after the initial palpation. The presence of the virus was demonstrated in the peripheral blood leucocytes of all eight calves at nine weeks. These results indicated that routine rectal palpation may be an effective mode of spread of bovine leukosis virus in susceptible cattle.