Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells
Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells
复制标题
传染性脾肾坏死病毒在鲈鱼脑细胞中高效复制需要加速有氧糖酵解和磷酸戊糖途径的代谢水平
DOI：
10.3390/biom9090440
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发表时间：
2019-09-01
期刊：
影响因子：
5.5
通讯作者：
Fu, Xiaozhe
中科院分区：
文献类型：
--
作者：
Guo, Xixi;Wu, Shiwei;Fu, Xiaozhe
葡萄糖是病毒增殖的主要碳源和能量来源，通常参与糖酵解、戊糖磷酸途径（PPP）和三羧酸循环（TCA循环）途径。在本研究中，我们研究了葡萄糖相关代谢途径在传染性脾肾坏死病毒（ISKNV）复制过程中的作用，该病毒给中华鲈鱼养殖业造成了严重的经济损失。通过转录组学、蛋白质组学和代谢组学的综合分析，我们发现ISKNV感染增强了ISKNV感染周期早期PPP和TCA循环的代谢途径，增强了ISKNV感染周期后期糖酵解途径。先进的结果证明，ISKNV复制在ISKNV感染周期后期诱导好氧糖酵解上调，而好氧糖酵解是ISKNV增殖所必需的。此外，提供核苷酸生物合成的PPP也是ISKNV增殖所必需的。然而，涉及葡萄糖的TCA循环对ISKNV增殖并不重要，而且是必要的。本文报道的结果为研究病毒代谢转变的发病机制以及抗病毒治疗策略提供了新的见解。
Glucose is a main carbon and energy source for virus proliferation and is usually involved in the glycolysis, pentose phosphate pathway (PPP), and tricarboxylic acid cycle (TCA cycle) pathways. In this study, we investigated the roles of glucose-related metabolic pathways during the replication of infectious spleen and kidney necrosis virus (ISKNV), which has caused serious economic losses in the cultured Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection enhanced the metabolic pathways of the PPP and the TCA cycle at the early stage of the ISKNV infection cycle and enhanced the glycolysis pathway at the late stage of the ISKNV infection cycle though the comprehensive analysis of transcriptomics, proteomics, and metabolomics. The advanced results proved that ISKNV replication induced upregulation of aerobic glycolysis at the late stage of ISKNV infection cycle and aerobic glycolysis were required for ISKNV multiplication. In addition, the PPP, providing nucleotide biosynthesis, was also required for ISKNV multiplication. However, the TCA cycle involving glucose was not important and necessary for ISKNV multiplication. The results reported here provide new insights into viral pathogenesis mechanism of metabolic shift, as well as antiviral treatment strategies.