Identification of Candidate Circular RNAs Underlying Intramuscular Fat Content in the Donkey.
Identification of Candidate Circular RNAs Underlying Intramuscular Fat Content in the Donkey.
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DOI：
10.3389/fgene.2020.587559
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发表时间：
2020
影响因子：
3.7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Li B;Feng C;Zhu S;Zhang J;Irwin DM;Zhang X;Wang Z;Zhang S
肌内脂肪（IMF）含量是衡量肉品品质的重要指标。环状RNA（circRNA）是参与许多生理过程的一大类内源性RNA。然而，circRNA在驴IMF中的表达和功能仍然没有得到解决。在这里，我们对驴背最长肌中的circRNA进行了表达谱分析，并鉴定了12，727个候选circRNA。其中，70%来自蛋白质基因的外显子。此外，在高（H）和低（L）IMF含量组中总共鉴定了127个差异表达（DE）circRNA，包括63个上调和64个下调的circRNA。DE circRNA的宿主基因的基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径富集分析显示宿主基因富集脂质代谢相关的GO术语（例如，使用酰基-CoA脱氢酶和MLL 3/4复合物的脂肪酸β-氧化），和信号传导途径（例如，TGF-β和赖氨酸降解信号通路）。进一步分析表明，有127个DE circRNA可能与miRNA相互作用，从而构建了circRNA-miRNA调控网络。多个circRNA可以潜在地充当调节脂肪细胞分化的miRNA的海绵。我们的研究结果提供了有价值的表达谱信息的circRNA在驴和新的见解circRNA的调控机制的调节IMF的内容。
Intramuscular fat (IMF) content is a crucial indicator of meat quality. Circular RNAs (circRNAs) are a large class of endogenous RNAs that are involved in many physiological processes. However, the expression and function of circRNA in IMF in the donkey remains unresolved. Here we performed an expression profiling of circRNAs in the donkey longissimus dorsi muscle and identified 12,727 candidate circRNAs. Among these, 70% were derived from the exons of protein genes. Furthermore, a total of 127 differentially expressed (DE) circRNAs were identified in high (H) and low (L) IMF content groups, including 63 upregulated and 64 downregulated circRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the host genes of the DE circRNAs showed that the host genes were enriched in lipid metabolism related GO terms (e.g., fatty acid beta-oxidation using acyl-CoA dehydrogenase and MLL3/4 complex), and signaling pathways (e.g., TGF-beta and lysine degradation signaling pathway). Further analyses indicated that 127 DE circRNAs were predicted to potentially interact with miRNAs, leading to the construction of circRNA-miRNA regulatory network. Multiple circRNAs can potentially function as sponges of miRNAs that regulate the differentiation of adipocytes. Our results provide valuable expression profile information for circRNA in the donkey and new insight into the regulatory mechanisms of circRNAs in the regulation of IMF content.