3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood.
3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood.
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DOI：
10.1007/s10544-010-9485-3
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发表时间：
2011-02
影响因子：
2.8
通讯作者：
Tai YC
中科院分区：
文献类型：
--
作者：
Zheng S;Lin HK;Lu B;Williams A;Datar R;Cote RJ;Tai YC
循环肿瘤细胞的检测已经成为转移性癌症患者的一种有前途的微创诊断和预后工具。我们报告了一种新的三维微过滤装置，可以从血液中富集活的循环肿瘤细胞。该装置由两层聚对二甲苯膜组成，其孔和间隙通过光刻法精确限定。孔的位置在顶部膜和底部膜之间移动。底膜支持捕获的细胞，并使细胞膜上的应力浓度最小化，并在过滤期间维持细胞活力。用扫描电子显微镜、共聚焦显微镜和免疫荧光染色，使用掺入血液或盐水中的培养肿瘤细胞的模型系统，研究装置上的活细胞捕获。本文提出并验证了这种新的3D微滤概念用于循环肿瘤细胞富集应用。该装置提供了一种非常有价值的工具，用于在研究和临床环境中评估和表征活的富集循环肿瘤细胞。
Detection of circulating tumor cells has emerged as a promising minimally invasive diagnostic and prognostic tool for patients with metastatic cancers. We report a novel three dimensional microfilter device that can enrich viable circulating tumor cells from blood. This device consists of two layers of parylene membrane with pores and gap precisely defined with photolithography. The positions of the pores are shifted between the top and bottom membranes. The bottom membrane supports captured cells and minimize the stress concentration on cell membrane and sustain cell viability during filtration. Viable cell capture on device was investigated with scanning electron microscopy, confocal microscopy, and immunofluorescent staining using model systems of cultured tumor cells spiked in blood or saline. The paper presents and validates this new 3D microfiltration concept for circulation tumor cell enrichment application. The device provides a highly valuable tool for assessing and characterizing viable enriched circulating tumor cells in both research and clinical settings.
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影响因子：
45.3
作者：
Cristofanilli, M;Hayes, DF;Terstappen, LWMM
通讯作者：
Terstappen, LWMM
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
11.5
作者：
Hayes, Daniel F.;Cristofanilli, Massimo;Terstappen, Leon W. W. M.
通讯作者：
Terstappen, Leon W. W. M.
DOI：
10.1097/00022744-200112000-00010
发表时间：
2001-12-01
影响因子：
1.6
作者：
Hoetelmans, RWM;Prins, FA;van Dierendonck, JH
通讯作者：
van Dierendonck, JH
影响因子：
3.8
作者：
Kahn, HJ;Presta, A;Marks, A
通讯作者：
Marks, A