Human interferon-λ3 is a potent member of the type III interferon family
Human interferon-λ3 is a potent member of the type III interferon family
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DOI：
10.1038/gene.2008.87
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发表时间：
2009-03-01
影响因子：
5
通讯作者：
Hartmann, R.
中科院分区：
文献类型：
--
作者：
Dellgren, C.;Gad, H. H.;Hartmann, R.
III型干扰素是新近发现的一个干扰素家族，它通过与I型干扰素相同的下游转录因子发出信号，但使用由IL-10R2和独特的干扰素-lambda R1受体链组成的单独的受体复合体。我们建立了一种简单高效的表达系统，以获得高纯度和高活性的三种人干扰素-lambda亚型(干扰素-lambda 1、-lambda 2和-lambda 3)，并利用该系统比较了不同干扰素-lambda亚型的生物学活性。令人惊讶的是，我们发现干扰素-lambda 3在人干扰素-lambda亚型中具有最高的比活性，比干扰素-lambda 1高2倍，比干扰素-lambda 2高16倍。此外，与商业上可用的干扰素-lambda 1和-lambda 2制剂相比，我们发现我们的干扰素-lambda制剂的活性更好。
Type III interferon (IFN) or IFN-lambda is a recently discovered family of IFNs that signal through the same downstream transcription factors as type I IFN but use a separate receptor complex composed of the IL-10R2 and the unique IFN-lambda R1 receptor chains. We have established a simple and efficient expression system to produce highly pure and active IFN-lambda of the three human IFN-lambda isoforms (IFN-lambda 1, -lambda 2 and -lambda 3) and used this to compare the biological activity of the different IFN-lambda subtypes. Surprisingly, we found IFN-lambda 3 to possess the highest specific activity of the human IFN-lambda subtypes, exhibiting a twofold higher activity than IFN-lambda 1 and a 16-fold higher activity than IFN-lambda 2. Furthermore, in comparison with the commercially available preparations of IFN-lambda 1 and -lambda 2, we found our IFN-lambda preparation to be superior in activity.