Determination of bacterial antibiotic resistance based on osmotic shock response.

Determination of bacterial antibiotic resistance based on osmotic shock response.
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基于渗透休克反应的细菌抗生素耐药性测定。

DOI:
10.1021/ac900968r
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发表时间:
2009
影响因子:
7.4
通讯作者:
Manalis,ScottR
Manalis,ScottR
中科院分区:
化学1区
文献类型:
--
作者:
Knudsen,ScottM;vonMuhlen,MarcioG;Schauer,DavidB;Manalis,ScottR

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We investigate the buoyant mass of bacterial cells in real time with the suspended microchannel resonator (SMR) as the population recovers from an osmotic shock. The density of the culture medium is chosen such that the bacteria initially have a positive buoyant mass which becomes negative as they recover from the hyperosmotic stress. This behavior can be used to differentiate between an antibiotic-resistant and an antibiotic-susceptible strain of the pathogenic bacteriaCitrobacter rodentium, and we propose a general approach for exploiting the high precision of the SMR for rapid detection of antibiotic resistance.
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