Injuring neurons induces neuronal differentiation in a population of hippocampal precursor cells in culture.
Injuring neurons induces neuronal differentiation in a population of hippocampal precursor cells in culture.
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损伤的神经元会诱导培养的海马前体细胞群中的神经元分化。
DOI：
10.1016/j.nbd.2005.10.007
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Dichter,MarcA
中科院分区：
文献类型：
--
作者：
Tseng,HenryC;Ruegg,StephanJ;Maronski,Margaret;Messam,ConradA;Grinspan,JudithB;Dichter,MarcA
海马前体细胞（HPC），可以诱导分化为星形胶质细胞和少突胶质细胞的新的人口可以从海马培养生长在无血清培养基。HPC是PDGF-反应性的，不与bFGF一起增殖，并且作为细胞片生长而不是聚集成神经球。HPC与少突胶质细胞前体细胞（OPC）共享许多标志物（A2 B5、GD 3、多唾液酸化神经元共同粘附分子（PSA-NCAM）和NG 2）。HPC不表达成熟神经元、星形胶质细胞或少突胶质细胞的标志物。与OPCs一样，在添加骨形态发生蛋白-4（BMP-4）和三碘甲状腺原氨酸（T3）的情况下，HPCs分别分化为胶质纤维酸性蛋白（GFAP）+星形胶质细胞和GalC+少突胶质细胞。加入或不加入碱性成纤维细胞生长因子（bFGF）、脑源性神经营养因子（BDNF）或视黄酸（RA），它们不会分化成神经元。这些HPC可以通过诱导附近培养的神经元中的神经元损伤或细胞死亡或通过来自损伤的神经元培养物的条件培养基来刺激以分化成神经元样细胞。在这些条件下，HPC生长更大，发展更广泛的树突状突起，成为微管相关蛋白-2-免疫反应，表达大的电压依赖性钠电流，并形成突触连接。内源性多能前体细胞在局部脑损伤后向神经元的转化可能是中枢神经系统稳态的重要组成部分。
A novel population of hippocampal precursor cells (HPCs) that can be induced to differentiate into astrocytes and oligodendrocytes can be derived from hippocampal cultures grown in serum-free media. The HPCs are PDGF-responsive, do not proliferate with bFGF, and grow as sheets of cells rather than gathering into neurospheres. The HPCs share many markers (A2B5, GD3, poly-sialylated neuronal common adhesion molecule (PSA-NCAM), and NG2) with oligodendrocyte precursor cells (OPCs). The HPCs do not express markers for mature neurons, astrocytes, or oligodendrocytes. Like OPCs, the HPCs differentiate into glial fibrillary acidic protein (GFAP)+ astrocytes and GalC+ oligodendrocytes with the addition of bone morphogenetic protein-4 (BMP-4) and triiodothyronine (T3), respectively. They do not differentiate into neurons with the addition or withdrawal of basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), or retinoic acid (RA). These HPCs can be stimulated to differentiate into neuron-like cells by the induction of neuronal injury or cell death in nearby cultured neurons or by conditioned medium from injured neuronal cultures. Under these conditions, HPCs grow larger, develop more extensive dendritic processes, become microtubule-associated protein-2-immunoreactive, express large voltage-dependent sodium currents, and form synaptic connections. The conversion of endogenous pluripotent precursor cells into neurons in response to local brain injury may be an important component of central nervous system homeostasis.