Study of a new bone-targeting titanium implant-bone interface.
Study of a new bone-targeting titanium implant-bone interface.
复制标题
新型骨靶向钛种植体-骨界面的研究。
DOI：
10.2147/ijn.s119520
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发表时间：
2016
影响因子：
8
通讯作者：
Lai R
中科院分区：
文献类型：
--
作者：
Liu X;Zhang Y;Li S;Wang Y;Sun T;Li Z;Cai L;Wang X;Zhou L;Lai R
骨靶向钛（Ti）种植体-骨界面的新策略，需要加强骨再生和骨整合的骨科和牙科种植体，特别是在骨质疏松症的科目。本研究通过将四环素接枝辛伐他汀（SV）聚合物胶束负载于二氧化钛纳米管（TNT）阵列中，成功构建了一种新型的双控、局部、骨靶向给药系统，并通过体内植入成功构建了骨靶向钛种植体-骨界面。在体外和体内评价生物学效应。结果表明，钛表面与TNT骨靶向胶束可以促进细胞骨架的扩展，早期粘附，碱性磷酸酶活性，和细胞外骨钙素浓度的大鼠成骨细胞，伴随着骨形态发生蛋白（BMP）-2的蛋白表达增强。在正常和去卵巢大鼠中建立单壁骨缺损种植体模型，作为绝经后骨质疏松症模型。显微计算机断层扫描成像和BMP-2在体内的表达表明，植入物与TNT靶向胶束表面能够促进骨再生和骨整合在两种动物模型。因此，无论是在体外还是在体内，这表明胶束的骨靶向作用大大提高了SV在种植体-骨界面上的生物利用度，并且单独提供载SV的靶向胶束显示出广泛应用于改善局部骨再生和骨整合的潜力，特别是在骨质疏松受试者中。
New strategies involving bone-targeting titanium (Ti) implant–bone interface are required to enhance bone regeneration and osseointegration for orthopedic and dental implants, especially in osteoporotic subjects. In this study, a new dual-controlled, local, bone-targeting delivery system was successfully constructed by loading tetracycline-grafted simvastatin (SV)-loaded polymeric micelles in titania nanotube (TNT) arrays, and a bone-targeting Ti implant–bone interface was also successfully constructed by implanting the delivery system in vivo. The biological effects were evaluated both in vitro and in vivo. The results showed that Ti surfaces with TNT–bone-targeting micelles could promote cytoskeletal spreading, early adhesion, alkaline phosphatase activity, and extracellular osteocalcin concentrations of rat osteoblasts, with concomitant enhanced protein expression of bone morphogenetic protein (BMP)-2. A single-wall bone-defect implant model was established in normal and ovariectomized rats as postmenopausal osteoporosis models. Microcomputed tomography imaging and BMP-2 expression in vivo demonstrated that the implant with a TNT-targeting micelle surface was able to promote bone regeneration and osseointegration in both animal models. Therefore, beneficial biological effects were demonstrated both in vitro and in vivo, which indicated that the bone-targeting effects of micelles greatly enhance the bioavailability of SV on the implant–bone interface, and the provision of SV-loaded targeting micelles alone exhibits the potential for extensive application in improving local bone regeneration and osseointegration, especially in osteoporotic subjects.