Determination of benzoxazinone derivatives in plants by combining pressurized liquid extraction-solid-phase extraction followed by liquid chromatography-electrospray mass spectrometry.
Determination of benzoxazinone derivatives in plants by combining pressurized liquid extraction-solid-phase extraction followed by liquid chromatography-electrospray mass spectrometry.
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加压液相萃取-固相萃取-液相色谱-电喷雾质谱联用测定植物中的苯并恶嗪酮衍生物。
DOI：
10.1021/jf050897p
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发表时间：
2006
影响因子：
6.1
通讯作者：
D. Barceló
中科院分区：
文献类型：
--
作者：
M. Villagrasa;M. Guillamon;E. Eljarrat;D. Barceló
评价了一种新的分析方法，该方法基于使用加压液体萃取（PLE），然后使用LiChrolut RP C18柱进行固相萃取，用于8种天然存在的苯并恶嗪酮衍生物的样品制备、萃取和净化，2-β-D-吡喃葡萄糖氧基-4-羟基-1，4-苯并恶嗪-3-酮、2-β-D-吡喃葡萄糖氧基-4-羟基-7-甲氧基-1，4-苯并恶嗪-3-酮、4-二羟基-1，4-苯并恶嗪-3-酮（DIBOA）、2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮、2-羟基-1，4-苯并恶嗪-3-酮、2-羟基-7-甲氧基-1，4-苯并恶嗪-3-酮、苯并恶唑啉-2-酮和6-甲氧基苯并恶唑啉-2-酮。然后，采用液相色谱-电喷雾质谱法，采用选择离子监测模式和内标（2-MeO-DIBOA、吲哚酚-β-D-葡萄糖苷和槲皮素-3-O-芸香糖苷）定量方法。本文证明了PLE方法的有效性，结合灵敏和具体的质谱检测，用于从植物中定量回收苯并恶嗪酮类化合物。分析物的回收率范围为66%至110%，变异系数范围为1%至14%。该方法的检测限在1至27微克/克之间。将该方法应用于3个不同小麦品种的叶和根中，检测范围为11-3261 μ g/g干重。
A new analytical method based on the use of pressurized liquid extraction (PLE) followed by solid-phase extraction with LiChrolut RP C18 cartridges was evaluated for the sample preparation, extraction, and cleanup of eight naturally occurring benzoxazinone derivatives, 2-beta-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one, 2-beta-D-glucopyranosyloxy-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one, 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one, 2-hydroxy-1,4-benzoxazin-3-one, 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one, benzoxazolin-2-one, and 6-methoxybenzoxazolin-2-one in plant samples. Afterward, liquid chromatography-electrospray mass spectrometry, using the selected ion monitoring mode and internal standard (2-MeO-DIBOA, indoxyl-beta-D-glucoside, and quercetin-3-O-rutinoside) quantification method was performed. This paper demonstrates the effectiveness of the PLE method, in conjunction with sensitive and specific mass spectrometric detection, for the quantitative recovery of compounds of the benzoxazinone class from plants. The recoveries of the analytes ranged from 66 to 110% with coefficients of variation ranging from 1 to 14%. This method gave detection limits between 1 and 27 microg/g. The method was applied to foliage and roots of three different wheat cultivars, and the analytes were detected in the range of 11-3261 microg/g of dry weight.