The effects of neuronal induction on gene expression profile in bone marrow stromal cells (BMSC)—a preliminary study using microarray analysis
The effects of neuronal induction on gene expression profile in bone marrow stromal cells (BMSC)—a preliminary study using microarray analysis
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DOI：
10.1016/j.brainres.2006.02.127
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发表时间：
2006-05
期刊：
影响因子：
2.9
通讯作者：
S. Yamaguchi;S. Kuroda;Hiroyuki Kobayashi;H. Shichinohe;S. Yano;K. Hida;K. Shinpo;S. Kikuchi;Y. Iwasaki
中科院分区：
文献类型：
--
作者：
S. Yamaguchi;S. Kuroda;Hiroyuki Kobayashi;H. Shichinohe;S. Yano;K. Hida;K. Shinpo;S. Kikuchi;Y. Iwasaki
骨髓基质细胞（BMSC）已被预期作为细胞类型的移植治疗在各种神经系统疾病的供体。然而，他们的神经原性能力仍然没有确定。在这项研究中，我们的目的是澄清是否在体外化学治疗促进其神经元分化的基因表达水平。用添加DMSO、视黄酸和碱性成纤维细胞生长因子的培养基培养小鼠BMSC，并对其形态和神经元标志物的表达进行评价。随后，使用微阵列和RT-PCR技术，分析处理诱导的基因表达谱变化。暴露于培养基后，BMSC模拟神经元样外观，并增加其对巢蛋白和Tuj-1的免疫反应性。微阵列分析显示，BMSC本身表达多谱系细胞基因，包括与神经元相关的基因。化学处理显著降低了间充质细胞相关基因的表达，并增加了5个神经元相关基因的表达。微阵列和RT-PCR分析也表明BMSC表达包括NGF-β和BDNF在内的多种生长因子的基因，表明它们在保护受损的中枢神经系统中的治疗作用。目前的结果表明，至少BMSC的某个亚群有可能根据周围环境改变其基因表达谱，并可能通过分泌神经保护因子来保护宿主组织。
Bone marrow stromal cells (BMSC) have been anticipated as a donor for cell type for transplantation therapy in various neurological disorders. However, their neurogenic capacity still remains undetermined. In this study, we aimed to clarify whether in vitro chemical treatment promotes their neuronal differentiation on the level of gene expression. Mice BMSC were cultured with medium supplemented with DMSO, retinoic acid, and basic fibroblast growth factor, and their morphology and expression of neuronal markers were evaluated. Subsequently, using microarray and RT-PCR techniques, the treatment-induced changes in the gene expression profile were analyzed. After exposure to the medium, the BMSC simulated a neuron-like appearance and increased their immunoreactivity for nestin and Tuj-1. Microarray analysis revealed that the BMSC per se express the multilineage cellular genes, including those associated with the neuron. Chemical treatment significantly decreased the expression of genes related to mesenchymal cells and increased the expression of 5 neuron-associated genes. Microarray and RT-PCR analyses also demonstrated that the BMSC express the genes for several growth factors including NGF-β and BDNF, indicating their therapeutic role in protecting the injured central nervous system. The present results suggest that at least a certain subpopulation of the BMSC have the potential to alter their gene expression profile in response to the surrounding environment and may possibly protect the host tissue by secreting neuroprotective factors.