Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota
Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota
复制标题
协同纤维素分解微生物群动态纤维素降解过程的比较宏基因组发现
DOI：
10.1007/s10570-020-03671-z
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发表时间：
2021
期刊：
影响因子：
5.7
通讯作者：
Li Xianzhen
中科院分区：
文献类型：
--
作者：
Yang Ming;Zhao Jingjing;Yuan Yue;Chen Xiaoyi;Yang Fan;Li Xianzhen
为了揭示纤维素降解的动态过程，发掘更多的纤维素酶，培养了一株具有纤维素降解能力的微生物群（FPDM），并对滤纸降解的不同阶段进行了比较。离子色谱分析和比较宏基因组测序结果表明，FPDM的多样性随着水解产物长度多样性的增加而增强，在纤维素降解的早中期和中晚期，芽孢噬菌体和Cohnelladenosine分别起着协同降解的主导作用。此外，推测363个下降移位和231个进行性移位未注释的基因分别参与纤维素到纤维糊精/纤维寡糖和纤维二糖的催化。根据酶解产物、群落结构和基因丰度的动态变化，预测了FPDM的动态纤维素降解途径。本研究为后续纤维素降解关键菌株和酶的鉴定以及纤维素降解机理的阐明提供了新的视角。
To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability was cultivated, and different stages of filter paper degradation were compared. Ion chromatography and comparative metagenomic sequencing revealed that the diversity of FPDM enhanced as the hydrolysate length diversity increased.SporocytophagaandCohnelladynamically dominated the synergistic degradation of cellulose in early-intermediate and intermediate-final periods, respectively. Moreover, 363 declining shifting and 231 progressive shifting unannotated genes were speculated to participate in the catabolism of cellulose to cellodextrin/cello-oligosaccharide and to cellobiose, respectively. Based on the dynamic changes in hydrolysates, community structure and gene abundance, a dynamic cellulose-degrading pathway of FPDM was predicted. Our work should provide a new perspective for subsequent identification of key cellulolytic strains and enzymes and clarification of the mechanism of cellulose biodegradation.