ESTIMATING INTRACELLULAR CONDUCTIVITY TENSORS FROM CONFOCAL MICROSCOPY OF RABBIT VENTRICULAR TISSUE
ESTIMATING INTRACELLULAR CONDUCTIVITY TENSORS FROM CONFOCAL MICROSCOPY OF RABBIT VENTRICULAR TISSUE
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DOI：
10.1515/bmt-2013-4333
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发表时间：
2013-08-01
影响因子：
1.7
通讯作者：
Doessel, O.
中科院分区：
文献类型：
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作者：
Bauer, S.;Edelmann, J-C;Doessel, O.
心脏的Bidomain模拟需要经过验证的参数来产生真实的数据。因此，有必要开发方法来估计这些参数的可靠值。我们开发了一种方法来提供这样的价值观，通过设计一个硅模型的细胞内电传导的基础上共聚焦显微镜数据的兔心室组织。使用高分辨率图像数据来确定心肌中的电导率的各向异性，其高度依赖于特定的组织几何形状。用不同光谱的荧光染料标记间隙连接蛋白connexin 43和细胞外间隙。将肌细胞分段，并提取肌细胞之间的差距连接密度。假设细胞内液体的电导率和间隙连接电阻，进行数值场计算的三个主要方向，以提取细胞内电导率张量。我们通过改变+/-50%的假设电导率来计算9个张量。我们分别估计了三个主要方向的细胞内电导率sigma（i，x）= 0：0653 S/m，sigma（i，y）= 0：0042 S/m和sigma（i，z）= 0：0033 S/m。估计的电导率值是现实的电各向异性，但需要改进，以适应其他实验数据。
Bidomain simulations of the heart need validated parameters to produce realistic data. Therefore, it is necessary to develop methods to estimate reliable values for these parameters. We developed an approach to deliver such values by designing an in-silico model of intracellular electrical conduction based on confocal microscopic data of rabbit ventricular tissue. High resolution image data were used to determine the anisotropy of electrical conductivity in the myocardium, which is highly dependent on the specific tissue geometry. Gap junction protein connexin43 and extracellular space were labeled with fluorescent dyes of different spectra. The myocytes were segmented and the gap junction density in-between myocytes was extracted. Assuming conductivities for intracellular liquid and gap junction resistance, a numerical field calculation was performed for three principal directions in order to extract intracellular conductivity tensors. We calculated 9 tensors by varying the assumed conductivities by +/- 50%. We estimated the intracellular conductivities for the three principal directions sigma(i,x) = 0:0653 S/m, sigma(i,y) = 0:0042 S/m and sigma(i,z) = 0:0033 S/m, respectively. The estimated conductivity values were realistic regarding the electrical anisotropy but need to be improved to fit other experimental data.