Polymorphisms of decoy receptor 3 are associated with risk of esophageal squamous cell carcinoma in Chinese Han
Polymorphisms of decoy receptor 3 are associated with risk of esophageal squamous cell carcinoma in Chinese Han
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诱饵受体3多态性与中国汉族食管鳞状细胞癌风险相关
DOI：
10.1007/s13277-010-0053-5
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发表时间：
2010-10-01
期刊：
影响因子：
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通讯作者：
Bai, Yun
中科院分区：
文献类型：
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作者：
Xiong, Gang;Guo, Hong;Bai, Yun
诱骗受体3（decoy receptor 3，DcR3）是一种可溶性受体，其肽段中不含跨膜和胞内序列。它可以结合并抑制凋亡诱导配体FasL、LIGHT和TL1A。本研究的目的是对食管鳞癌患者和对照组DcR3基因启动子区的两个多态性位点进行基因分型，分析个体遗传变异与食管鳞癌易感性的关系。共444名中国ESCC患者和468名匹配的非癌症对照者评估了DCR 3的两个单核苷酸多态性（SNP）。使用SNPShot技术对多态性进行基因分型。采用逆转录-聚合酶链反应（RT-PCR）检测癌组织中DCR3的表达。ESCC组和对照组中两种SNPs的等位基因分布差异有显著性（P <0.01）。与147 TT基因型相比，147 CC基因型与ESCC风险显著升高相关（比值比，1.89; 95%可信区间，1.30 - 2.96）。147CC基因型在吸烟者中的风险（优势比，2.19; 95%可信区间，1.32 - 3.64）比在非吸烟者中的风险（优势比，1.25; 95%可信区间，0.59 - 2.69）更显著。分层分析发现，147例CC与临床TNM分期有显著相关性（P <0.01）。不同147 C/T基因型的食管鳞癌组织中DCR 3的表达差异有统计学意义。提示DcR3基因启动子区147 C/T多态性可能通过影响DcR3的表达而影响中国汉族人群食管鳞癌的易感性。
Decoy receptor 3 (DcR3) is a soluble receptor without transmembrane and intracellular sequences in its peptide. It can bind to and inactivate the apoptosis-inducing ligand FasL, LIGHT, and TL1A. The aims of this study are to genotype the two polymorphisms in the promotor of DcR3 in esophageal squamous cell cancer patients and controls and analyze the association between individual genetic variation and susceptibility to esophageal squamous cell carcinoma (ESCC). A total of 444 Chinese ESCC patients and 468 matched cancer-free controls were evaluated for the two single nucleotide polymorphisms (SNPs) in DCR3. Polymorphisms were genotyped using the SNPShot technique. The expression of DCR3 in cancer tissues was detected by reverse transcription-polymerase chain reaction. There were significant differences in the allele's distribution of the two SNPs among ESCC cases and controls (P< 0.01). Compared with the 147TT genotype, the 147CC genotype was associated with significant elevated risk of ESCC (odds ratio, 1.89; 95% confidence interval, 1.30–2.96). The risk of 147CC genotype was more notable (odds ratio, 2.19; 95% confidence interval, 1.32–3.64) in the smokers than in the nonsmokers (odds ratio, 1.25; 95% confidence interval, 0.59–2.69). In the stratification analyses, we also found there was a strong correlation between 147 CC and the clinical TNM stage (P< 0.01). Furthermore, significant difference in DCR3 expression in ESCC tissues was found between subgroups with different 147C/T variant. Our finding suggested that DcR3 promoter polymorphism 147C/T might influence the susceptibility to ESCC in the Chinese Han population, maybe by influencing DcR3 expression.