Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation
Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation
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DOI：
10.1111/j.1478-3231.2008.01911.x
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发表时间：
2009-04-01
影响因子：
6.7
通讯作者：
Levkau, Bodo
中科院分区：
文献类型：
--
作者：
Baba, Hideo A.;Wohlschlaeger, Jeremias;Levkau, Bodo
Survivin调节细胞分裂，抑制细胞凋亡。肝脏再生是一个复杂的过程，涉及细胞增殖和细胞凋亡。survivin的作用尚未得到很好的阐明，在人类中也没有相关数据。采用70%肝切除法观察大鼠肝再生。通过逆转录酶聚合酶链反应、免疫印迹和免疫组织化学鉴定Survivin。定量观察细胞增殖和细胞凋亡。对33例活体供肝移植患者进行肝活检，研究移植后17天内的存活免疫表达、增殖和凋亡情况。7名健康捐献者作为对照。再生24-72 h后，大鼠肝细胞中Survivin转录物和蛋白表达水平显著上调，且与细胞增殖呈显著相关，而与细胞凋亡无显著相关性。在人类中，供体和再灌注肝组织中几乎不存在survivin，但在移植后5-7天显著增加，且与增殖相关，而与凋亡无关。Survivin在人类和啮齿类动物肝脏再生中表达上调，并与增殖相关，表明Survivin与细胞分裂有关。
Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as controls.Survivin transcript and protein were significantly upregulated in rat hepatocytes after 24-72 h during regeneration and showed a significant correlation with proliferation but not with apoptosis. In humans, survivin was nearly absent in donor and reperfused liver tissue but increased significantly 5-7 days after transplantation and correlated with proliferation but not with apoptosis.Survivin is upregulated in human and rodent liver regeneration and correlates with proliferation, suggesting an association of survivin and cell division.