Effect of DEC1 on the proliferation, adhesion, invasion and epithelial-mesenchymal transition of osteosarcoma cells
Effect of DEC1 on the proliferation, adhesion, invasion and epithelial-mesenchymal transition of osteosarcoma cells
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DOI：
10.3892/etm.2020.8459
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发表时间：
2020-03-01
影响因子：
2.7
通讯作者：
Liu, Cong-Wei
中科院分区：
文献类型：
--
作者：
Li, Shuai;Peng, Dan;Liu, Cong-Wei
分化的胚胎软骨细胞表达基因1（DEC 1）与各种类型的人类癌症有关，然而，关于DEC 1在骨肉瘤中的功能的数据有限。本研究旨在检测DEC 1在人骨肉瘤组织和细胞系中的表达。进一步研究DEC 1对骨肉瘤细胞增殖、粘附、侵袭和上皮间质转化（EMT）的影响。逆转录-定量PCR和western blot检测结果显示，DEC 1在人骨肉瘤组织和骨肉瘤细胞系中的表达水平均高于对照组。功能获得和丧失实验均表明，DEC 1在体外促进骨肉瘤细胞的增殖、粘附和侵袭，分别通过MTT、细胞粘附和细胞侵袭试验确定。此外，发现DEC 1上调间充质标志物N-钙粘蛋白和波形蛋白，同时下调上皮标志物E-钙粘蛋白。总之，本研究表明，在人骨肉瘤组织和细胞系中，DEC 1的表达水平增加，并确定DEC 1可能通过促进细胞增殖、粘附和侵袭来发挥其在骨肉瘤进展中的作用。此外，DEC 1被证明对骨肉瘤细胞系中的EMT具有诱导作用，从而有助于骨肉瘤细胞的侵袭性。这项初步研究表明，DEC 1可能作为一个新的分子靶点，用于治疗骨肉瘤。
Differentiated embryonic chondrocyte-expressed gene 1 (DEC1) is associated with various types of human cancer; however, there is limited data regarding the functions of DEC1 in osteosarcoma. The present study aimed to examine the expression of DEC1 in human osteosarcoma tissues and cell lines. Furthermore, the effects of DEC1 on the proliferation, adhesion, invasion and epithelial-mesenchymal transition (EMT) of osteosarcoma cells were investigated. Using reverse transcription-quantitative PCR and western blot analysis, it was found that the expression levels of DEC1 were higher in human osteosarcoma tissues and osteosarcoma cell lines than in the controls. Both gain- and loss-of-function experiments suggested that DEC1 promotes the proliferation, adhesion and invasion of osteosarcoma cells in vitro, as determined by MTT, cell adhesion and cell invasion assays, respectively. Additionally, DEC1 was found to upregulate the mesenchymal markers N-cadherin and vimentin, whilst downregulating the epithelial marker E-cadherin. In conclusion, this present study showed increased expression levels of DEC1 in human osteosarcoma tissues and cell lines, and identified that DEC1 may exert its effect on osteosarcoma progression by promoting cell proliferation, adhesion and invasion. Furthermore, DEC1 was shown to have an inducible effect on EMT in osteosarcoma cell lines, thus contributing to the aggressiveness of osteosarcoma cells. This initial study indicated that DEC1 may serve as a novel molecular target for the treatment of osteosarcoma.