Automatic measurements of hydrogen peroxide utilizing a Xylenol Orange-titanium system
Automatic measurements of hydrogen peroxide utilizing a Xylenol Orange-titanium system
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DOI：
10.1021/ac60258a023
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发表时间：
1968-02
影响因子：
7.4
通讯作者：
C. D. Nordschow;A. Tammes
中科院分区：
文献类型：
--
作者：
C. D. Nordschow;A. Tammes
Otomo（/）描述了使用二甲酚橙-过氧化氢体系测量钛作为三聚体螯合物的第三反应物的方法。现有的分光光度证据（1-3）表明，螯合物在每个成分中都是等摩尔的。该系统，原则上，应同样适用于过氧化氢测定。我们需要在几种含蛋白质的复杂生物溶液中，如动物血清、血浆和悬浮代谢细胞菌落的Eagles修饰和未修饰培养基中，以微摩尔浓度对过氧化氢进行快速选择和重复分析。我们无法使用某些程序进行自动连续流分析，因为它们缺乏特异性(4)，或者它们依赖于浓缩试剂，而浓缩试剂的特异性约束已经令人满意地显示出来（56）。因此，我们修改了Otomo(1)所描述的钛测定方法，并使其适应于连续流系统。在该系统中，透析是处理含蛋白溶液所必需的，但在不需要透析的情况下则不需要透析。
The use of a xylenol orange-hydrogen peroxide system to measure titanium as the third reactant of a trimeric chelate, has been described by Otomo (/). Available spectrophotometric evidence (1-3) suggests the chelate to be equimolar in each constituent. The system, in principle, should be equally adaptable to hydrogen peroxide assay. We required a rapid selective and repetitive analysis of hy-drogen peroxide, at micromole concentrations, in several protein-containing complex biological solutions, eg, animal serums, plasmas, and Eagles’ modified and unmodified culture media in which were suspended metabolizing cell colonies. We were unable to use certain procedures for automatic con-tinuous flow analysis because of their lack of specificity (4) or their dependence upon concentrated reagents where the con-straint of specificity has been satisfactorily shown (56). We therefore modified the titanium assay method described by Otomo (1) and adapted it to a continuous flow system. In this system dialysis is required to process protein-containing solutions but would be unnecessarywhere this requisite does not obtain.