Novel immunohistochemical markers differentiate intrahepatic cholangiocarcinoma from benign bile duct lesions
Novel immunohistochemical markers differentiate intrahepatic cholangiocarcinoma from benign bile duct lesions
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DOI：
10.1136/jclinpath-2015-203418
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发表时间：
2016-07-01
影响因子：
3.4
通讯作者：
Baba, Hideo A.
中科院分区：
文献类型：
--
作者：
Bertram, Stefanie;Padden, Juliet;Baba, Hideo A.
目的 肝内胆管癌 (ICC) 和良性胆管病变之间的区别可能具有挑战性。使用我们之前确定的 ICC 潜在生物标志物，我们通过免疫组织化学方法检查了这些生物标志物是否有助于鉴别诊断 ICC、胆管腺瘤和反应性胆管增殖，并确定了包括已知生物标志物的诊断标志物组。 方法受试者包括来自 77 名 ICC 患者、33 名胆管腺瘤患者和 47 名肝硬化胆管反应患者的样本。我们之前鉴定的生物标志物（应激诱导磷蛋白 1 (STIP1)、SerpinH1、14-3-3Sigma）与文献中的候选物（分化簇 56、热休克蛋白 (HSP) 27、HSP70、B 细胞淋巴瘤 2、p53、ki67）进行比较后进行了免疫组织化学测试。结果 SerpinH1 和 14-3-3Sigma 的表达在ICC 高于胆管腺瘤和胆管反应（p5%，可用于区分恶性和非恶性病变。
Aims The distinction between intrahepatic cholangiocarcinoma (ICC) and benign bile duct lesions can be challenging. Using our previously identified potential biomarkers for ICC, we examined whether these are useful for the differential diagnosis of ICC, bile duct adenoma and reactive bile duct proliferations in an immunohistochemical approach and identified a diagnostic marker panel including known biomarkers.Methods Subjects included samples from 77 patients with ICC, 33 patients with bile duct adenoma and 47 patients with ductular reactions in liver cirrhosis. Our previously identified biomarkers (stress-induced phosphoprotein 1 (STIP1), SerpinH1, 14-3-3Sigma) were tested immunohistochemically following comparison with candidates from the literature (cluster of differentiation 56, heat shock protein (HSP) 27, HSP70, B-cell-lymphoma2, p53, ki67).Results The expression of SerpinH1 and 14-3-3Sigma was significantly higher in ICC than in bile duct adenomas and ductular reactions (p5% might be used for the distinction of malignant and non-malignant lesions.