Protein crystallography with spallation neutrons: collecting and processing wavelength‐resolved Laue protein data
Protein crystallography with spallation neutrons: collecting and processing wavelength‐resolved Laue protein data
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使用散裂中子进行蛋白质晶体学：收集和处理波长分辨劳厄蛋白质数据
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发表时间：
2004
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通讯作者：
Gayle Greene
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作者：
P. Langan;Gayle Greene
洛斯阿拉莫斯中子科学中心的蛋白质晶体学站是第一个在散裂中子源上建造的站。飞行时间方法与大型电子位置敏感和时间敏感探测器相结合，用于收集波长分辨的劳厄衍射数据。波长分辨劳厄技术对于蛋白质晶体学来说是一项新技术，需要开发新的数据收集和数据分析策略。 d*TREK 软件套件经过改造，与 Daresbury Laue 软件套件、仪器控制软件 PCS-GUI 和图形程序 O 结合使用，用于在蛋白质晶体学站波长分辨 Laue 环境中收集和处理数据。给出了典型的红氧还蛋白、胰岛素和 d-木糖异构酶数据处理示例，单位晶胞范围为 34.32 × 35.31 × 44.23 A 到 93.78 × 88.53 × 102.90 A，从而说明了仪器的功能和仪器软件的范围。
The protein crystallography station at Los Alamos Neutron Science Center is the first to be built at a spallation neutron source. Time-of-flight methods in combination with a large electronic position-sensitive and time-sensitive detector are used in order to collect wavelength-resolved Laue diffraction data. The wavelength-resolved Laue technique is new to protein crystallography and has required the development of new strategies for data collection and data analysis. The software suite d*TREK has been adapted and used in combination with the Daresbury Laue software suite, the instrument control software PCS-GUI, and the graphics program O, for data collection and processing in the protein crystallography station wavelength-resolved Laue environment. Examples are given, as typical, for data processing from rubredoxin, insulin and d-xylose isomerase with unit cells ranging from 34.32 × 35.31 × 44.23 A to 93.78 × 88.53 × 102.90 A, thus illustrating the power of the instrument and the scope of the instrument software.