IMMUNOCHEMICAL AND IMMUNOFLUORESCENCE STUDIES OF BETA‐TRACE PROTEIN IN DIFFERENT SPECIES AND ORGANS, WITH SPECIAL REFERENCE TO THE CENTRAL NERVOUS SYSTEM
IMMUNOCHEMICAL AND IMMUNOFLUORESCENCE STUDIES OF BETA‐TRACE PROTEIN IN DIFFERENT SPECIES AND ORGANS, WITH SPECIAL REFERENCE TO THE CENTRAL NERVOUS SYSTEM
复制标题
不同物种和器官中β-痕量蛋白的免疫化学和免疫荧光研究，特别是中枢神经系统
DOI：
10.1111/j.1471-4159.1973.tb06007.x
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发表时间：
1973
影响因子：
4.7
通讯作者：
L. Nord
中科院分区：
文献类型：
--
作者：
J. Olsson;L. Nord
-用免疫化学和免疫荧光方法研究了β-痕量蛋白在人类不同器官和其他哺乳动物大脑中的定位。利用双向免疫扩散技术，可以证实人脑脊液、脑、股神经、睾丸、附睾体、精索、卵巢、输卵管和猴脑中β-示踪蛋白的免疫同一性。通过琼脂单径向免疫扩散，在脑和生殖器官的水溶性蛋白制剂中，β-痕量蛋白的含量最高，尤其是在白色中枢神经系统物质、睾丸和附睾中。用间接免疫荧光技术观察到中枢神经系统，尤其是白质和附睾间质有较强的荧光。股神经中的β-微量蛋白含量明显低于中枢神经系统。结果表明，β-示踪蛋白与中枢神经系统之间可能存在联系，可能在髓鞘或神经胶质细胞中，也可能与生殖系统之间存在联系，可能与参与类固醇代谢的细胞有关。对于高度发育的哺乳动物，即人和猴子，提出了β-微量蛋白的特异性，因为当小牛、兔子或小鼠的脑提取液与抗人β-微量蛋白的抗血清孵育时，没有发现沉淀。
—The localization of beta‐trace protein in different human organs and in the brains of other mammals has been investigated with immunochemical and immunofluorescence methods. With the double immunodiffusion technique, the immunological identity between beta‐trace protein in human CSF, brain, femoral nerve, testis, epididymis, spermatic cord, ovary and fallopian tube and in monkey brain could be demonstrated. By means of single radial immunodiffusion on agar, the highest concentrations of beta‐trace protein were found in preparations of water‐soluble proteins of brain and genital organs, especially in white CNS matter, testis and epididymis. With the indirect immunofluorescence technique, bright fluorescence was found in CNS, especially in white matter, and in the stroma of epididymis. The content of beta‐trace protein in femoral nerve was found to be considerably lower than that in the CNS. The results indicate a connexion between beta‐trace protein and the CNS, probably in the myelin or glial cells, and also between beta‐trace protein and the genital system, possibly to cells involved in steroid metabolism. A specificity for highly developed mammals, i.e. man and monkey, is proposed for beta‐trace protein, as no precipitation could be found when brain extracts from calves, rabbits or mice were incubated with anti‐serum against human beta‐trace protein.