Cassava C-repeat binding factor 1 gene responds to low temperature and enhances cold tolerance when overexpressed in Arabidopsis and cassava
Cassava C-repeat binding factor 1 gene responds to low temperature and enhances cold tolerance when overexpressed in Arabidopsis and cassava
复制标题
木薯 C 重复结合因子 1 基因在拟南芥和木薯中过表达时，可响应低温并增强耐冷性。
DOI：
10.1007/s11103-017-0596-6
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发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Zhang, Peng
中科院分区：
文献类型：
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作者：
An, Dong;Ma, Qiuxiang;Zhang, Peng
低温是影响全球重要作物生存、生产力和地理分布的主要非生物胁迫因素。C-repeat/dehydration-responsive elements binding transcription factors（CBF/DREB）是植物非生物胁迫应答的重要调节因子。在这项研究中，MeCBF 1，CBF-like基因，被确定在热带块根作物木薯（木薯）。MeCBF 1编码的蛋白质与拟南芥以及其他物种的DREB 1As/CBFs具有很强的同源性。MeCBF 1定位于细胞核，主要在茎和成熟叶中表达，而在顶芽和茎形成层中不表达。MeCBF 1的表达不仅对低温有高度的响应，而且在盐、PEG和阿坝处理下也有显著的诱导作用。在其启动子区发现了几个胁迫相关的顺式元件，例如，ABRE相关、MYC识别位点和MYB应答元件。与AtCBF 1相比，MeCBF 1在木薯中被冷诱导的表达被延迟，并且仅在4 h后上调，这也通过其启动子活性得到证实。MeCBF 1在转基因拟南芥和木薯植株中的过表达赋予了增强的耐冷性。CBF调节子较小，并且在过表达的木薯中不完全与MeCBF 1表达共调节。MeCBF 1表达的延迟和CBF-regulon的减弱可能导致木薯对冷敏感。
Low temperature is a major abiotic stress factor affecting survival, productivity and geographic distribution of important crops worldwide. The C-repeat/dehydration-responsive element binding transcription factors (CBF/DREB) are important regulators of abiotic stress response in plants. In this study, MeCBF1, a CBF-like gene, was identified in the tropical root crop cassava (Manihot esculenta Crantz). The MeCBF1 encodes a protein that shares strong homology with DREB1As/CBFs from Arabidopsis as well as other species. The MeCBF1 was localized to the nucleus and is mainly expressed in stem and mature leaves, but not in apical buds or stem cambium. MeCBF1 expression was not only highly responsive to cold, but also significantly induced by salt, PEG and ABA treatment. Several stress-associated cis-elements were found in its promoter region, e.g., ABRE-related, MYC recognition sites, and MYB responsive element. Compared with AtCBF1, the MeCBF1 expression induced by cold in cassava was retarded and upregulated only after 4 h, which was also confirmed by its promoter activity. Overexpression of MeCBF1 in transgenic Arabidopsis and cassava plants conferred enhanced crytolerance. The CBF regulon was smaller and not entirely co-regulated with MeCBF1 expression in overexpressed cassava. The retarded MeCBF1 expression in response to cold and attenuated CBF-regulon might lead cassava to chilling sensitivity.