Modulation of astrocyte proliferation by HIV-1: Differential effects in productively infected, uninfected, and Nef-expressing cells
Modulation of astrocyte proliferation by HIV-1: Differential effects in productively infected, uninfected, and Nef-expressing cells
复制标题
DOI：
10.1016/j.jneuroim.2006.05.020
复制
发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Lee, Sunhee C.
中科院分区：
文献类型：
--
作者：
Cosenza-Nashat, Melissa A.;Si, Qiusheng;Lee, Sunhee C.
尽管反应性星形胶质细胞在正常大脑中处于静止状态，但它可以在各种疾病中增殖。我们研究了暴露于 VSVg env 假型 HIV-1（产生高水平感染）的培养物中 HIV-1 对星形胶质细胞增殖的影响。 HIV-1 在增加未感染 (p24-) 星形胶质细胞增殖的同时，强烈抑制有效感染 (p24+) 细胞的增殖。细胞周期停滞是 G1/S，而不是 G2/M（通常归因于 Vpr 的类型）。无法确定 Vpr 或 Nef 的明确作用。腺病毒介导的 Nef 表达（“限制性”感染模型）诱导星形胶质细胞的 M 期停滞。我们推测 HIV-1 是体内星形胶质细胞增殖的重要调节剂。 (c) 2006 Elsevier B.V. 保留所有权利。
Although quiescent in normal brain, reactive astrocytes can proliferate in various disorders. We examined the impact of HIV-1 on astrocyte proliferation in cultures exposed to VSVg env-pseudotyped HIV-1 which yields high levels of infection. HIV-1, while increasing the proliferation of uninfected (p24-) astrocytes, strongly inhibited proliferation of productively infected (p24+) cells. The cell cycle arrest was G1/S rather than G2/M, a type commonly attributed to Vpr. No clear role of Vpr or Nef could be identified. Adenovirus-mediated expression of Nef (a model of "restricted" infection) induced M-phase arrest of astrocytes. We speculate that HIV-1 is a significant modulator of astrocyte proliferation in vivo. (c) 2006 Elsevier B.V. All rights reserved.