Ethane exhalation as an index of in vivo lipid peroxidation: concentrating ethane from a breath collection chamber.
Ethane exhalation as an index of in vivo lipid peroxidation: concentrating ethane from a breath collection chamber.
复制标题
乙烷呼出作为体内脂质过氧化的指标：从呼吸收集室浓缩乙烷。
DOI：
10.1016/0003-2697(82)90282-2
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发表时间：
1982
影响因子：
2.9
通讯作者：
Cohen,G
中科院分区：
文献类型：
--
作者：
Lawrence,GD;Cohen,G
描述了一种从大量空气中浓缩乙烷的简单方法；检测呼出乙烷的灵敏度极限提高了100倍或更多。该方法用于监测体内组织脂质过氧化损伤引起的低水平乙烷呼出。测试了不同吸附剂对乙烷和乙烯的浓缩作用；此外，还研究了不同类型管道的渗透性特性，以构建适合实验动物的廉价呼吸收集室。推荐吸附剂为活性炭和分子套筒；活性氧化铝吸附剂性能差。聚氯乙烯和聚四氟乙烯管是不渗透的，而乳胶和硅胶管是渗透性的乙烷。当乙烷和乙烯被腹腔注射到小鼠体内时，这些挥发性碳氢化合物的呼出在呼吸收集室中很容易被监测到。虽然乙烯和c3 - c5烷烃可能会被小鼠代谢，但乙烷显然不会；然而，一部分乙烷似乎被人体组织吸收而滞留。
A simple method is described for concentrating ethane from large volumes of air; the sensitivity limits for detecting exhaled ethane are increased by 100-fold or more. The method is intended for monitoring low-level ethane exhalation resulting from peroxidative damage to tissue lipids in vivo. Various adsorbents for concentrating ethane and ethylene were tested; in addition, the permeability characteristics of various types of tubing were studied in order to construct a suitable, inexpensive breath collection chamber for laboratory animals. Recommended adsorbents are activated charcoal and molecular sleve; activated alumina is a poor adsorbent. Polyvinyl and Teflon tubing are impermeable, whereas latex and silicone tubing are permeable to ethane. When ethane and ethylene are injected intraperitoneally into mice, the exhalation of these volatile hydrocarbons is readily monitored in the breath collection chamber. Whereas ethylene and the C3–C5alkanes probably are metabolized by mice, ethane apparently is not; however, a portion of the ethane appears to be trapped by absorption within body tissues.