Parental genome imbalance in Brassica oleracea causes asymmetric triploid block
Parental genome imbalance in Brassica oleracea causes asymmetric triploid block
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甘蓝亲本基因组失衡导致不对称三倍体块
DOI：
10.1111/j.1365-313x.2012.05015.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Stoute A
中科院分区：
文献类型：
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作者：
Stoute A
在许多植物物种中，由于胚乳发育异常，倍性间杂交失败。在近亲繁殖的物种拟南芥中，父本和母本过量倍性杂交通常会产生对胚乳发育和最终种子大小表现出亲本起源效应的有活力的种子。父本过量杂交导致胚乳和较大种子的扩展增殖，而相反，母本过量杂交导致早期胚乳细胞化和较小种子。亲本基因剂量对种子发育影响的研究表明，MADS盒转录因子，特别是AGAMOUS样家族，在控制胚乳增殖中起重要作用。重要的作物拟南芥属包含自交不亲和的远交种，并且具有比密切相关的拟南芥更大和更复杂的基因组。在这里，我们表明，尽管甘蓝显示出强烈的亲本起源对种子发育的影响，三倍体块由于胚乳发育的致命破坏被限制在父本过剩，与母本过剩的杂交产生可行的种子。此外，对与亲本效应相关的拟南芥基因的拟南芥同源物的转录组分析揭示了两个物种中控制胚乳行为的某些机制的保守性。然而，也有差异，可能解释了失败的父本多余的交叉在甘蓝。
Interploidy crosses fail in many plant species due to abnormalities in endosperm development. In the inbreeding speciesArabidopsis thaliana, both paternal and maternal excess interploidy crosses usually result in viable seed that exhibit parent‐of‐origin effects on endosperm development and final seed size. Paternal excess crosses result in extended proliferation of the endosperm and larger seeds, while conversely maternal excess crosses result in early endosperm cellularisation and smaller seeds. Investigations into the effect of parental gene dosage on seed development have revealed that MADS box transcription factors, particularly the AGAMOUS‐like family, play important roles in controlling endosperm proliferation. The important crop genusBrassicacontains self‐incompatible outbreeding species and has a larger and more complex genome than the closely related Arabidopsis. Here we show that althoughBrassica oleraceadisplays strong parent‐of‐origin effects on seed development, triploid block due to lethal disruption of endosperm development was restricted to paternal excess, with maternal excess crosses yielding viable seed. In addition, transcriptome analyses ofBrassicahomologues of Arabidopsis genes linked to parent‐of‐origin effects revealed conservation of some mechanisms controlling aspects endosperm behaviour in the two species. However, there were also differences that may explain the failure of the paternal excess cross inB. oleracea.